{"title":"Immunostimulatory Activity of Solubilized Peptidoglycan Derivatives Prepared From Lactic Acid Bacteria","authors":"Sho Noguchi, Momoko Nakayama, Masahiro Eguchi, Sakura Onoue, Hiroaki Kouzai, Kazuyoshi Kawahara","doi":"10.1111/1348-0421.70025","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>To develop vaccine adjuvants from bacterial peptidoglycan (PG) the immunostimulatory activity of lysozyme-solubilized PG derived from <i>Levilactobacillus brevis</i> and <i>Lactiplantibacillus plantarum</i> was investigated. Solubilized PG from both bacteria induced IL-8 in THP-1 cells, and periodate oxidation of <i>L. plantarum</i> PG reduced the activity, suggesting that muramyl dipeptide was partially destroyed. Periodate-oxidized <i>L. plantarum</i> PG showed reduced IL-8 inducing activity in NOD2-expressing cells, while it remained in NOD1-expressing cells, suggesting that γ-<span>d</span>-glutamyl-<i>meso</i>-diaminopimelic acid structure was maintained. All PG preparations stimulated RAW264.7 cells to proliferate, suggesting that they could be potent candidates for vaccine adjuvants.</p>\n </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 1","pages":"47-51"},"PeriodicalIF":1.6000,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology and Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.70025","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
To develop vaccine adjuvants from bacterial peptidoglycan (PG) the immunostimulatory activity of lysozyme-solubilized PG derived from Levilactobacillus brevis and Lactiplantibacillus plantarum was investigated. Solubilized PG from both bacteria induced IL-8 in THP-1 cells, and periodate oxidation of L. plantarum PG reduced the activity, suggesting that muramyl dipeptide was partially destroyed. Periodate-oxidized L. plantarum PG showed reduced IL-8 inducing activity in NOD2-expressing cells, while it remained in NOD1-expressing cells, suggesting that γ-d-glutamyl-meso-diaminopimelic acid structure was maintained. All PG preparations stimulated RAW264.7 cells to proliferate, suggesting that they could be potent candidates for vaccine adjuvants.
为研制细菌肽聚糖（PG）疫苗佐剂，研究了短乳杆菌和植物乳杆菌溶菌酶溶肽聚糖的免疫刺激活性。两种细菌的溶解PG诱导THP-1细胞中的IL-8，植物乳杆菌PG的高碘酸氧化降低了活性，表明muramyl二肽被部分破坏。在表达nod2的细胞中，经高碘酸盐氧化的L. plantarum PG的IL-8诱导活性降低，而在表达nod1的细胞中，IL-8诱导活性保持不变，表明γ-d-谷氨酰胺-中二氨基戊酸结构得以维持。所有PG制剂都能刺激RAW264.7细胞增殖，表明它们可能是疫苗佐剂的有力候选物。
期刊介绍：
Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses.
Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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