[Method for determining infectious and hemagglutinating titer of influenza A/Mallard Pennsylvania/10218/84 (H5N2) virus by changing the microviscosity of the viral membrane after interaction with phospholipid modifiers using cholesterol free liposomes as an example].
N A Kontarov, I V Pogarskaya, E I Dolgova, E O Kontarova, V V Pomazanov, R R Gafarov, S G Mardanly, N V Yuminova
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Abstract
Introduction: The antiviral action of a number of drugs is associated with their modification of the lipid membrane of viruses. One of the possible mechanisms of such modification of the viral membrane is the extraction of cholesterol from the membranes of virions.
Objective of the study: A method has been developed for determining the infectious and hemagglutinating titer of avian influenza virus by changing the microviscosity of the viral membrane after incubation with phospholipid modifiers, using cholesterol-free liposomes consisting of phosphatidylcholine and phosphatidylethanolamine in a molar ratio of 1 : 2 for 48 hours as an example.
Materials and methods: The extraction process was confirmed by two methods: gel filtration with radioactively labeled liposomes and virions, and by changing the polarization value of the fluorescent probe 1-anilinonaphthalene-8-sulfonate anion (8-ANS) in the viral membrane.
Results: A correlation was found between the change in infectious and hemagglutinating titer and the microviscosity of the viral membrane.
Conclusion: In this regard, it seems possible to use this dependence to determine the infectious and hemagglutinating activity of the influenza virus within one serotype in clinical laboratory diagnostics, using various fluorescent probes. It should be noted that not only liposomes of a certain composition can be used as lipophilic modifiers of the viral membrane, but also such compounds as ethylene glycol, erythritol, glycerol.
期刊介绍：
The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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