One-step warming technique for vitrified human blastocysts using commercially available thawing solutions

IF 3.9 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2026-01-01 Epub Date: 2025-07-23 DOI:10.1016/j.rbmo.2025.105168

Rena Kato , Fumiaki Itoi , Aiko Tajima, Nozomi Oono, Kanae Mori, Sumika Watanabe, Rio Tanaka, Mao Akita, Hinano Touyama, Mao Ibata, Saki Nakamura, Mika Kamata, Yukio Hattori

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引用次数: 0

Abstract

Research question

What are the re-expansion rates and clinical outcomes obtained when employing a one-step warming technique using three commercially available thawing solutions on vitrified blastocysts, and how do they compare with the standard multi-step warming technique?

Design

This retrospective cohort study involved 1173 frozen embryo transfers (FET) in women aged <42 years between December 2022 and December 2024. The blastocysts were warmed following multi-step warming guidelines (with Reprolife solutions) or using one-step warming with 1 min of immersion in a thawing solution. Warmed blastocysts were cultured in a recovery medium for 2–3 h, and then transferred to the embryo transfer medium. Embryo transfer was performed after another 2 h. Survival, re-expansion and clinical outcomes were compared between 573 FET using one-step warming and 600 FET using multi-step warming, as well as between one-step warming using three different brands of thawing solution: Irvine (319 FET), Kitazato (128 FET) and Reprolife (126 FET).

Results

The re-expansion rate of blastocysts after 4–5 h was significantly higher with one-step warming (92.4%) compared with multi-step warming (86.8%) (P = 0.0009). The clinical pregnancy rate was also significantly higher with one-step warming (69.3%) compared with multi-step warming (61.2%) (P = 0.0042), but the ongoing pregnancy rate did not differ significantly between one-step warming (53.9%) and multi-step warming (49.2%). No significant differences in re-expansion or clinical outcomes were detected between the three different brands of thawing solution used for one-step warming.

Conclusions

One-step warming results in better re-expansion and clinical outcomes than multi-step warming, and one-step warming is equally effective with the three commercially available brands of thawing solution tested.

查看原文本刊更多论文

使用市售的解冻溶液对玻璃化的人类囊胚进行一步加热技术。

研究问题：使用三种市售解冻液对玻璃化囊胚采用一步加热技术获得的再膨胀率和临床结果如何？与标准的多步加热技术相比如何？设计：本回顾性队列研究涉及1173例老年女性冷冻胚胎移植（FET）。结果：一步加热后4-5 h囊胚再膨胀率（92.4%）显著高于多步加热后的再膨胀率（86.8%）（P = 0.0009）。临床妊娠率一步温（69.3%）显著高于多步温（61.2%）（P = 0.0042），但持续妊娠率一步温（53.9%）与多步温（49.2%）无显著差异。用于一步加热的三种不同品牌的解冻液在再膨胀或临床结果方面没有显著差异。结论：一步加热的再膨胀效果和临床效果优于多步加热，一步加热与三种市售解冻液的效果相同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.