Divanillin synthesis from vanillin by horseradish peroxidase in consideration of mathematical model and morphology of product

IF 3.6 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-03-01 Epub Date: 2025-11-24 DOI:10.1016/j.bej.2025.110016

Ikuya Teranishi, Shusuke Ito, Shintaro Morisada, Keisuke Ohto, Hidetaka Kawakita

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引用次数: 0

Abstract

Vanillin is one of the monomers originated from lignin. We have synthesized divanillin from vanillin as a precipitate using the enzymatic reaction of horseradish peroxidase (HRP), to examine the effects of the concentration of HRP and of the reaction medium on the reactivity of the substrate and the morphology of the product, divanillin. Mathematical modeling was used to analyze the effects of solvent-induced enzyme inactivation and substrate reactivity on substrate concentration. The amount of HRP on the reaction influenced the inactivation due to the reaction media. It was quantitatively shown that changing the concentration of methanol in the reaction medium increased the solvation to the substrate vanillin and increased the contact efficiency with HRP. SEM observation of the precipitates revealed that they were spherical and needle-like and varied with the reaction conditions. Divanillin is a substance with potential for subsequent effective utilization of biomass, and this research will lead to future applications of novel materials.

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从数学模型和产物形态的角度考虑，用辣根过氧化物酶由香兰素合成二万尼林

香兰素是源自木质素的单体之一。我们利用辣根过氧化物酶（HRP）的酶促反应，将香兰素作为沉淀物合成了万西林，考察了HRP浓度和反应介质对底物反应活性和产物万西林形态的影响。采用数学模型分析了溶剂诱导酶失活和底物反应性对底物浓度的影响。由于反应介质的不同，HRP的投加量会影响失活效果。定量结果表明，改变反应介质中甲醇的浓度可以提高对底物香兰素的溶剂化，提高与HRP的接触效率。对析出物的SEM观察表明，析出物呈针状球形，随反应条件的变化而变化。vananillin是一种具有后续有效利用生物质潜力的物质，该研究将导致未来新材料的应用。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.