First report of Fusarium equiseti and Fusarium oxysporum causing brown leaf spot on Chinese cabbage

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2026-04-01 Epub Date: 2025-11-21 DOI:10.1016/j.cropro.2025.107484

Ran Gu , Changjiang Wang , Jingjing Ji , Xianfeng Zhang , Fengxin Shi , Yan Liu , Yaowei Zhang

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引用次数: 0

Abstract

Chinese cabbage (Brassica rapa subsp. pekinensis), a leafy vegetable of Chinese origin, is widely cultivated in East Asia. During August–September 2023, an outbreak of brown leaf spot was observed on Chinese cabbage at Xiangyang Farm, Xiangfang District, Harbin, China. Initial symptoms on infected plants included round to irregular brown leaf spots with whorled centers, which gradually expanded and eventually led to complete leaf necrosis. The causal agents were identified through isolation, morphological characterization, and phylogenetic analysis based on the internal transcribed spacer (ITS), elongation factor 1-α (EF1-α) gene, and the second largest subunit of RNA polymerase II (RPB2). The pathogens were identified as Fusarium equiseti (F. equiseti) and Fusarium oxysporum (F. oxysporum). Koch's postulates were fulfilled by inoculating pure cultures onto Chinese cabbage, where symptoms induced by F. equiseti were consistent with those observed in the field. Host range assays demonstrated that F. equiseti can infect plants from Poaceae, Fabaceae, Solanaceae, Brassicaceae, Cucurbitaceae and Amaranthaceae. Biological characterization showed that F. equiseti (F.eq 1) exhibited optimal growth at 25 °C and pH 7 under any photoperiod, using a nitrogen-free medium supplemented with carbon sources including soluble starch, sodium carboxymethyl cellulose (CMC-Na), or xylose. To our knowledge, this is the first report of F. equiseti and F. oxysporum causing brown leaf spot on Chinese cabbage in China and worldwide.

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大白菜褐叶斑病的镰刀菌和尖孢镰刀菌初报

大白菜（芸苔）原产于中国的一种叶菜，在东亚广泛种植。2023年8 - 9月，在中国哈尔滨市香坊区向阳农场发现大白菜发生褐叶斑病。感染植株的初始症状包括圆形到不规则的棕色叶斑，中心呈轮状，逐渐扩大，最终导致叶片完全坏死。基于内部转录间隔段（ITS）、延伸因子1-α (EF1-α)基因和RNA聚合酶II （RPB2）第二大亚基，通过分离、形态学鉴定和系统发育分析确定了致病因子。病原鉴定为马尖镰刀菌（Fusarium equiseti）和尖孢镰刀菌（Fusarium oxysporum）。通过将纯培养物接种到大白菜上，证实了科赫的假设，在大白菜上，马瘟菌引起的症状与田间观察到的症状一致。寄主范围测定表明，该病菌可侵染禾科、豆科、茄科、十字花科、葫芦科和苋科植物。生物学特性研究表明，在25°C和pH为7的无氮培养基中，添加可溶性淀粉、羧甲基纤维素钠（CMC-Na）或木糖等碳源，在任何光周期下，F. equiseti （F.eq 1）生长最佳。据我们所知，这是中国和世界上第一次报道引起大白菜褐叶斑病的F. equiseti和F. oxysporum。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.