Structure and gene cluster of the capsular polysaccharide of Acinetobacter baumannii K104

IF 3.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-12 DOI:10.1016/j.carres.2025.109745

Anna M. Shpirt , Zakhar V. Serpokrylov , Andrei V. Perepelov , Andrei S. Dmitrenok , Mikhail M. Shneider , Anastasiya V. Popova , Philip V. Toukach , Yuriy A. Knirel

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引用次数: 0

Abstract

Structure of the Acinetobacter baumannii MAR20-4513 (K104) capsular polysaccharide (CPS) was established using Smith degradation, ¹H and ¹³C NMR spectroscopy, including two-dimensional homonuclear ¹Н,¹Н COSY, TOCSY, ROESY and heteronuclear ¹Н,¹³C HSQC and HMBC experiments, and modeling. The K104 CPS is composed of a branched pentasaccharide K unit containing two d-Glcp residues, and one residue each of d-GalpNAc, d-GlcpNAc and d-Galp. The following structure of the pentasaccharide repeating unit of the CPS was established.

Functions of genes in the K locus of A. baumannii K104 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.

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鲍曼不动杆菌K104荚膜多糖的结构与基因簇。

采用Smith降解、1H和13C NMR谱法建立鲍曼不动杆菌MAR20-4513 （K104）荚膜多糖（CPS）的结构，包括二维同核1Н、1Н COSY、TOCSY、ROESY和异核1Н，13C HSQC和HMBC实验，并建模。K104 CPS由一个支链五糖K单元组成，其中包含两个d-Glcp残基，d-GalpNAc、d-GlcpNAc和d-Galp各一个残基。建立了CPS的五糖重复单元的结构如下：鲍曼不动杆菌K104 K位点的基因功能与现有数据库的序列比较，发现与CPS结构一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".