Quercetin-loaded MgO nanoparticles in a chitosan/gelatin/PVA matrix enhance KGF1 expression and accelerate wound healing.

IF 2.5 4区医学 Q3 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-17 DOI:10.1177/08853282251399589

Najmeh Darvishi, Somayeh Reiisi, Sadegh Shirian

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引用次数: 0

Abstract

Effective treatment of skin wounds is essential due to the skin's protective, regulatory, and aesthetic functions. Post-injury infections can significantly impair healing, highlighting the need for advanced biomaterials that combine antimicrobial activity with regenerative potential. In this study, we developed a multifunctional chitosan/gelatin/polyvinyl alcohol (CS/GEL/PVA) nanocomposite containing magnesium oxide (MgO) nanoparticles loaded with quercetin (MgO@QC), aimed at enhancing wound healing and promoting keratinocyte growth factor 1 (KGF1) expression. MgO nanoparticles were synthesized and characterized using DLS, zeta potential, FTIR, XRD, FESEM, and TEM. Quercetin was successfully loaded onto the MgO nanoparticles with a high loading efficiency of 99%, as confirmed by spectroscopic analyses. The resulting nanocomposite demonstrated favorable physicochemical properties, including uniform morphology, excellent swelling behavior (∼79%), optical clarity, and robust structural integrity. Hemolysis assays revealed excellent hemocompatibility, while in vitro cytotoxicity tests confirmed biocompatibility up to 500 µg/mL. Cell proliferation and migration assays (MTT and scratch test) showed dose-dependent enhancement of fibroblast activity, particularly at 1 mg/mL. The nanocomposite also significantly upregulated KGF1 gene expression, suggesting its role in stimulating epithelial regeneration. In vivo studies using a murine excisional wound model demonstrated accelerated wound closure and tissue regeneration in the MgO@QC-treated group, supported by histological evidence of angiogenesis, re-epithelialization, and reduced inflammation. The CS/GEL/PVA/MgO@QC nanocomposite offers a biocompatible and bioactive platform that significantly enhances wound healing. These findings suggest its strong potential for clinical application as an advanced wound dressing for acute and chronic skin injuries.

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壳聚糖/明胶/PVA基质中负载槲皮素的MgO纳米颗粒可增强KGF1的表达并加速伤口愈合。

由于皮肤的保护、调节和审美功能，有效治疗皮肤伤口是必不可少的。损伤后感染会严重影响愈合，因此需要结合抗菌活性和再生潜力的先进生物材料。在这项研究中，我们开发了一种多功能壳聚糖/明胶/聚乙烯醇（CS/GEL/PVA）纳米复合材料，其中含有氧化镁（MgO）纳米颗粒，负载槲皮素（MgO@QC），旨在促进伤口愈合和促进角质细胞生长因子1 （KGF1）的表达。采用DLS、zeta电位、FTIR、XRD、FESEM和TEM等方法对MgO纳米颗粒进行了表征。槲皮素被成功地装载到MgO纳米颗粒上，其装载效率高达99%，经光谱分析证实。所得到的纳米复合材料表现出良好的物理化学性能，包括均匀的形态、优异的溶胀性（~ 79%）、光学清晰度和坚固的结构完整性。溶血试验显示其具有良好的血液相容性，体外细胞毒性试验证实其生物相容性可达500µg/mL。细胞增殖和迁移试验（MTT和划痕试验）显示成纤维细胞活性的剂量依赖性增强，特别是在1mg /mL时。纳米复合材料还显著上调KGF1基因表达，提示其在刺激上皮再生中的作用。使用小鼠切除伤口模型进行的体内研究表明，MgO@QC-treated组伤口愈合和组织再生加速，组织学证据支持血管生成、再上皮化和炎症减少。CS/GEL/PVA/MgO@QC纳米复合材料提供了生物相容性和生物活性平台，显著增强伤口愈合。这些发现表明其作为急性和慢性皮肤损伤的高级伤口敷料具有很强的临床应用潜力。

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来源期刊

Journal of Biomaterials Applications 工程技术-材料科学：生物材料

CiteScore

5.10

自引率

3.40%

发文量

144

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials. Peer-reviewed articles by biomedical specialists from around the world cover: New developments in biomaterials, R&D, properties and performance, evaluation and applications Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices Current findings in biological compatibility/incompatibility of biomaterials The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use. The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.