T. L. Nemirovskaya, K. A. Zaripova, S. P. Belova, K. A. Sharlo, D. A. Sidorenko
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引用次数: 0
Abstract
The hypothesis of the relationship between changes in the calcium throughput of dihydropyridine receptors (DHPR) and the accumulation of ATP and phosphoinositide 3-kinases (PI3) in the muscle during its unloading was checked. We also checked the PI3K signaling pathway involvement in the atrophic process regulation in skeletal muscles during their unloading. To do this, we inhibited DHPR and PI3K with nifedipine and with LY294002. We found that both sarcolemma-related units are involved in the regulation of ATP content, calcium signaling markers, and IP3 levels in the unloaded m. soleus. DHPR inhibition prevents ATP, Ca2+, and IP3 accumulation in soleus under unloading. PI3K inhibition reduces the degree of atrophy, ATP and IP3 content in muscle, and regulates the Ca-dependent signaling markers under tree day soleus unloading.
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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