The potential ameliorative effect of curcumin nanoemulsion on busulfan-induced testicular toxicity in adult rat model

IF 3.4 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2026-01-01 Epub Date: 2025-11-10 DOI:10.1016/j.reprotox.2025.109106

Sarah E. Amin , Eman F. Farghaly , Doaa A. Abdelmonsif , Eiman I. Zaki , Walaa Omar

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引用次数: 0

Abstract

Many factors contribute to infertility. Among these are chemotherapeutic drugs as they interfere with cell division and spermatogenesis. Busulfan (BU) is one of these drugs that is applied in the treatment of many malignancies. Latest studies have focused on the protective impact of medicinal plant products, such as curcumin (CU), for ameliorating tissue damage through their antioxidant properties. Nanoparticles are now used to improve drug delivery and efficacy. Our study was aimed at assessing the potential therapeutic effect of curcumin nanoemulsion (CU-NE) on busulfan-induced testicular toxicity in adult rats. Forty-eight adult male albino rats were randomly distributed in six groups, 8 rats each: Control groups; group I: negative control, II: vehicle control, III: CU-NE. Group IV: BU, group V: CU-NE+BU and group VI: spontaneous recovery. Each group was assessed regarding serum level of testosterone, tumor necrosis factor-α (TNF-α) and total antioxidant capacity (TAC). Malondialdehyde (MDA) and glutathione peroxidase (GPX) levels were measured in testicular tissue. Moreover, sperm parameters were measured, and histological examination of the testis was performed. CU-NE significantly improved all the tested parameters and ameliorated the degenerative changes found in the testis following busulfan administration. Thus, CU-NE could be a promising therapeutic agent that restores spermatogenesis and facilitates regeneration of the seminiferous epithelium after chemotherapy-induced testicular damage.

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姜黄素纳米乳对布苏芬所致成年大鼠睾丸毒性的潜在改善作用。

许多因素导致不孕。其中包括化疗药物，因为它们会干扰细胞分裂和精子发生。布磺胺（BU）是其中一种用于治疗多种恶性肿瘤的药物。最近的研究集中在药用植物产品的保护作用，如姜黄素（CU），通过其抗氧化特性来改善组织损伤。纳米颗粒现在被用于改善药物传递和疗效。本研究旨在评估姜黄素纳米乳（CU-NE）对丁硫丹诱导的成年大鼠睾丸毒性的潜在治疗作用。48只成年雄性白化大鼠随机分为6组，每组8只：对照组；ⅰ组为阴性对照组，ⅱ组为载药对照组，ⅲ组为CU-NE组。IV组：BU， V组：CU-NE+BU， VI组：自发恢复。评估各组血清睾酮水平、肿瘤坏死因子-α (TNF-α)和总抗氧化能力（TAC）。测定睾丸组织中丙二醛（MDA）和谷胱甘肽过氧化物酶（GPX）水平。此外，测量精子参数，并对睾丸进行组织学检查。CU-NE显著改善了所有测试参数，并改善了布苏凡给药后睾丸的退行性改变。因此，CU-NE可能是一种很有前景的治疗药物，可以在化疗引起的睾丸损伤后恢复精子发生并促进精细胞上皮的再生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.