Different metabolic paradigms and distribution of regulatory T cells between primary and lymph node metastasis prostate cancer.

IF 2.2 4区医学 Q2 PATHOLOGY

Cytojournal Pub Date : 2025-09-08 eCollection Date: 2025-01-01 DOI:10.25259/Cytojournal_44_2025

Wangli Mei, Shuai Liu, Mengyu Wei, Shiyong Xin, Xiang Liu

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引用次数: 0

Abstract

Objective: The objectives of the study are to investigate the differential metabolic paradigms and distribution of regulatory T (Tregs) cells between primary prostate cancer (PCa) and lymph node (LN) metastases.

Material and methods: Single-cell RNA sequencing analysis of primary PCa and LN metastases was employed to reveal the immune infiltration, identify Treg cell clusters, and analyze their metabolic regulation. Immunohistochemical (IHC) for FOXP3 and cluster of differentiation antigen 45 was used to verify different distribution and infiltration of Treg cells.

Results: Immune cell infiltration was prominent around PCa cells, with Tregs significantly enriched in node-positive samples, suggesting an immunosuppressive microenvironment. Three Treg subsets were identified: Inhibitory Tregs, effector Tregs, and double-positive Tregs, each exhibiting distinct metabolic profiles. IHC confirmed higher Treg infiltration in LN metastases compared to primary tumors, particularly within tumor stroma.

Conclusion: Tregs promote lymphatic metastasis in PCa through metabolic reprogramming, with their infiltration levels serving as a potential biomarker for metastatic risk.

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原发性前列腺癌和淋巴结转移性前列腺癌的不同代谢模式和调节性T细胞分布。

目的：研究原发性前列腺癌（PCa）和淋巴结（LN）转移的差异代谢模式和调节性T （Tregs）细胞的分布。材料与方法：采用原发性PCa和LN转移灶的单细胞RNA测序分析，揭示免疫浸润，鉴定Treg细胞簇，分析其代谢调控。采用免疫组化法（IHC）检测FOXP3和分化抗原簇45，验证Treg细胞的分布和浸润情况。结果：前列腺癌细胞周围有明显的免疫细胞浸润，淋巴结阳性标本中Tregs显著富集，提示存在免疫抑制微环境。鉴定出三种Treg亚群：抑制性Treg、效应Treg和双阳性Treg，每一种都表现出不同的代谢特征。免疫组化证实，与原发肿瘤相比，淋巴结转移瘤中Treg的浸润更高，尤其是在肿瘤基质中。结论：Tregs通过代谢重编程促进前列腺癌的淋巴转移，其浸润水平可作为转移风险的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytojournal PATHOLOGY-

CiteScore

2.20

自引率

42.10%

发文量

审稿时长

>12 weeks

期刊介绍： The CytoJournal is an open-access peer-reviewed journal committed to publishing high-quality articles in the field of Diagnostic Cytopathology including Molecular aspects. The journal is owned by the Cytopathology Foundation and published by the Scientific Scholar.