Investigation of the Effect of Quercetin on the Accumulation of Ciprofloxacin Resistance in Staphylococcus aureus.

IF 2.3 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2026-12-01 Epub Date: 2025-11-10 DOI:10.1177/15353141251394587

Haorong Chen, Xiaofeng Luo, Panpan Liu, Jingge Wang, Junpeng Huang, Guiqin Wang

{"title":"Investigation of the Effect of Quercetin on the Accumulation of Ciprofloxacin Resistance in Staphylococcus aureus.","authors":"Haorong Chen, Xiaofeng Luo, Panpan Liu, Jingge Wang, Junpeng Huang, Guiqin Wang","doi":"10.1177/15353141251394587","DOIUrl":null,"url":null,"abstract":"Quinolones are one of the most widely used antibiotics globally for the treatment of Staphylococcus aureus infections, and resistance to their various generations of drugs occurs. The present study aimed to investigate the inhibitory effect of quercetin on quinolone-resistant S. aureus, triggered by efflux pumps, to assess its potential value as an efflux pump inhibitor. The minimum inhibitory concentration of ciprofloxacin for resistant bacteria was determined by the micro broth dilution method, combined with UV spectrophotometry and high-performance liquid chromatography, to observe the changes in ciprofloxacin accumulation in the antibiotic-resistant bacteria after treatment with quercetin, quantitative real-time PCR (qRT-PCR) was used to analyze the transcript levels of some efflux pump genes, finally, the whole proteins of S. aureus were extracted by the action of quercetin, following bicinchoninic acid (BCA) quantification, proteins were separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and overall changes in total protein bands were observed. The results showed that 64-256 µg/mL quercetin significantly increased the accumulation of ciprofloxacin in antibiotic-resistant bacteria, inhibited the efflux of ciprofloxacin by 2-10-fold downregulation of the expression of the efflux pump genes norA, norB, norC, and mepA, and modulated the protein expression profile of S. aureus. This study revealed that quercetin has the potential to act as an efflux pump inhibitor, providing a theoretical basis for the treatment of antibiotic-resistant bacterial infections.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"869-876"},"PeriodicalIF":2.3000,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1177/15353141251394587","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/11/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Quinolones are one of the most widely used antibiotics globally for the treatment of Staphylococcus aureus infections, and resistance to their various generations of drugs occurs. The present study aimed to investigate the inhibitory effect of quercetin on quinolone-resistant S. aureus, triggered by efflux pumps, to assess its potential value as an efflux pump inhibitor. The minimum inhibitory concentration of ciprofloxacin for resistant bacteria was determined by the micro broth dilution method, combined with UV spectrophotometry and high-performance liquid chromatography, to observe the changes in ciprofloxacin accumulation in the antibiotic-resistant bacteria after treatment with quercetin, quantitative real-time PCR (qRT-PCR) was used to analyze the transcript levels of some efflux pump genes, finally, the whole proteins of S. aureus were extracted by the action of quercetin, following bicinchoninic acid (BCA) quantification, proteins were separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and overall changes in total protein bands were observed. The results showed that 64-256 µg/mL quercetin significantly increased the accumulation of ciprofloxacin in antibiotic-resistant bacteria, inhibited the efflux of ciprofloxacin by 2-10-fold downregulation of the expression of the efflux pump genes norA, norB, norC, and mepA, and modulated the protein expression profile of S. aureus. This study revealed that quercetin has the potential to act as an efflux pump inhibitor, providing a theoretical basis for the treatment of antibiotic-resistant bacterial infections.

查看原文本刊更多论文

槲皮素对金黄色葡萄球菌耐环丙沙星积累影响的研究。

喹诺酮类药物是全球范围内用于治疗金黄色葡萄球菌感染的最广泛使用的抗生素之一，并且对其各代药物都存在耐药性。本研究旨在研究槲皮素对外排泵引发的喹诺酮类耐药金黄色葡萄球菌的抑制作用，以评估其作为外排泵抑制剂的潜在价值。采用微肉汤稀释法测定耐药菌对环丙沙星的最低抑制浓度，结合紫外分光光度法和高效液相色谱法，观察槲皮素处理后耐药菌体内环丙沙星积累的变化，采用实时荧光定量PCR （qRT-PCR）分析部分外排泵基因的转录本水平。槲皮素作用下提取金黄色葡萄球菌全蛋白，BCA定量，SDS-PAGE分离蛋白，考马斯亮蓝染色，观察总蛋白条带的整体变化。结果表明，64-256µg/mL槲皮素可显著增加耐药菌体内环丙沙星的积累，通过下调外排泵基因norA、norB、norC和mepA的2-10倍表达抑制环丙沙星的外排，并调节金黄色葡萄球菌的蛋白表达谱。本研究揭示槲皮素具有作为外排泵抑制剂的潜力，为耐药细菌感染的治疗提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.