Identification and QTL analysis of stripe rust resistance in the common wheat cultivar Gaoyuan813.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-11-05 eCollection Date: 2025-11-01 DOI:10.1007/s11032-025-01616-7

Kanghui Zeng, Yuqin Li, Liuyang Shang, Yanling Hu, Zhenzhen Wei, Qiang Zhou, Lianquan Zhang, Dengcai Liu, Bo Zhang, Lin Huang

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引用次数: 0

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to global wheat production. Developing resistant wheat varieties is a crucial objective in wheat breeding programs. The Chinese common wheat cultivar Gaoyuan813 (GY813) has exhibited excellent resistance to Pst under field conditions for several years. The objective of this study was to identify quantitative trait loci (QTLs) associated with stripe rust resistance using a recombinant inbred line (RIL) population derived from a cross between Gaoyuan813 and the susceptible variety Fukuho. The RILs were evaluated for stripe rust resistance in four field environments with a mixture of Chinese prevalent Pst races (CYR32, CYR33, CYR34, Zhong4, and HY46) and in a growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism array. Five QTLs for stripe rust resistances were mapped to chromosomes 1BL (2), 2AS (2), and 7DS (1), explaining 4.37%-25.44% of the phenotypic variance. QYrsicau-2AS.1 and QYrsicau-7DS were stably detected across all field environments, whereas QYrsicau-1BL.2 was only detected in the growth chamber test. QYrsicau-1BL.1 and QYrsicau-7DS may correspond to the known resistance genes Yr29 and Yr18, respectively, while QYrsicau-1BL.2 and QYrsicau-2AS.2 are likely novel. Additive effects were observed for the combination of QYrsicau-1BL.1, QYrsicau-2AS.1, and QYrsicau-7DS. KASP markers linked to QYrsicau-1BL.2 (KASP_AX-109878201) and QYrsicau-2AS.1 (KASP_AX-110981112) were developed and validated to facilitate the breeding use of genes for wheat improvement.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01616-7.

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普通小麦品种高远813抗条锈病的鉴定及QTL分析。

小麦条锈病是由小麦条锈病引起的小麦条锈病，是全球小麦生产的主要威胁之一。培育抗抗性小麦品种是小麦育种计划的一个重要目标。我国普通小麦品种高远813 （GY813）多年来在田间条件下表现出优异的抗Pst能力。以高远813与易感品种福昊杂交的重组自交系（RIL）群体为材料，研究了与抗条锈病相关的数量性状位点（qtl）。在中国流行的Pst小种（CYR32、CYR33、CYR34、Zhong4和HY46）混合的4种田间环境和CYR34小种的生长室中，利用Wheat55K单核苷酸多态性阵列进行基因分型，评估了ril对条锈病的抗性。5个与条锈病抗性相关的qtl定位在1BL(2)、2AS(2)和7DS(1)染色体上，解释了4.37% ~ 25.44%的表型变异。QYrsicau-2AS。qyrsicau -1和QYrsicau-7DS在所有野外环境中都能稳定检测到，而QYrsicau-1BL在所有野外环境中都能检测到。2只在生长室试验中检测到。QYrsicau-1BL。qyrsicau -1和QYrsicau-7DS可能分别对应于已知的抗性基因Yr29和Yr18，而QYrsicau-1BL。2和qrysicau - 2as。2种可能是新颖的。与QYrsicau-1BL联用观察到加性效应。1, QYrsicau-2AS。1、QYrsicau-7DS。与qyrsicau - 1bl连锁的KASP标记（KASP_AX-109878201）和QYrsicau-2AS.1（KASP_AX-110981112）为小麦改良基因的育种利用提供了便利。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01616-7。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.