Genetic dissection of plant height in spring wheat from Pakistan using genome-wide SNPs and allele-specific Rht markers.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-11-07 eCollection Date: 2025-11-01 DOI:10.1007/s11032-025-01600-1

Hafiz Muhammad Suleman, Humaira Qayyum, Sana Ur Rehman, Khawar Majeed, Misbah Mukhtar, Saima Zulfiqar, Zahid Mahmood, Abdul Aziz, Muhammad Fayyaz, Ambreen Mehvish, Shuanghe Cao, Awais Rasheed, Zhonghu He

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Abstract

Plant architecture and yield potential of wheat are significantly influenced by plant height (PH). In the present study, a diversity panel consisting of 199 Pakistani wheat cultivars was evaluated for PH in three environments, and a genome-wide association study (GWAS) was conducted to identify loci associated with reduced height. GWAS identified 19 loci associated with reduced height, of which 12 were consistently identified in all environments. Allelic variations were analyzed in the diversity panel for five Rht genes, including Rht-B1, Rht-D1, Rht13, Rht25, and Rht26, using diagnostic KASP markers. Furthermore, a KASP marker was developed to identify the dwarfing allele Rht-B1p in wheat. The GA-insensitive dwarfing allele Rht-B1b allelic frequency was pre-dominant (69.6%), followed by the GA-sensitive Rht26 mutant allele (58.5%). Five dwarfing alleles of Rht25, including Rht25c, Rht25d, and Rht25e were rarely present in the cultivars, with frequencies of 1.5%, 1%, and 0.5%, respectively. The use of alternate dwarfing alleles to reduce PH can increase the genetic base of wheat cultivars by reducing selection pressure on the Rht-B1b/Rht-D1b haplotype and can lead to the development of wheat cultivars with improved characteristics such as reduced lodging risk, increased resource allocation to grain, improved harvest efficiency, enhanced crop stability, and adaptability.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01600-1.

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利用全基因组snp和等位基因特异性Rht标记分析巴基斯坦春小麦株高。

小麦株高（PH）对植株构型和产量潜力有显著影响。在本研究中，研究人员对199个巴基斯坦小麦品种在3种环境下的PH值进行了评估，并进行了全基因组关联研究（GWAS），以确定与高度降低相关的位点。GWAS鉴定出19个与身高降低相关的位点，其中12个位点在所有环境中均被一致鉴定。利用诊断性KASP标记，在多样性面板上分析5个Rht基因（包括Rht- b1、Rht- d1、Rht13、Rht25和Rht26）的等位基因变异。在此基础上，建立了小麦矮化等位基因Rht-B1p的KASP标记。ga不敏感矮化等位基因Rht-B1b的等位基因频率为前显性（69.6%），其次是ga敏感突变等位基因Rht26（58.5%）。Rht25矮化等位基因Rht25c、Rht25d和Rht25e在品种中很少出现，频率分别为1.5%、1%和0.5%。利用交替矮化等位基因降低PH值，可以通过减轻Rht-B1b/Rht-D1b单倍型的选择压力，增加小麦品种的遗传基础，从而培育出具有降低倒伏风险、增加籽粒资源配置、提高收获效率、增强作物稳定性和适应性等性状的小麦品种。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01600-1。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.