Structure of the O-polysaccharide from the lipopolysaccharide of Vreelandella venusta strain B511

IF 3.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI:10.1016/j.carres.2025.109734

Maxim S. Kokoulin , Elena N. Sigida , Vlada S. Belova , Marina S. Kuzina , Natalya S. Velichko , Vyacheslav S. Grinev , Yuliya P. Fedonenko

引用次数: 0

Abstract

Lipopolysaccharide was obtained by hot aqueous-phenol extraction from Vreelandella venusta B511, a moderately halophilic Gram-negative bacterium isolated from mud of Lake Botkul, Volgograd Region, Russia. The O-polysaccharide (OPS) was released by mild acid hydrolysis of the lipopolysaccharide and was structurally characterized by chemical analyses, by 1D and 2D NMR and FTIR spectroscopy. The following structure of the OPS trisaccharide repeating unit was identified: →4)-β-D-ManpNAcA-(1→4)-β-D-ManpNAcA3Ac-(1→3)-α-D-GlcpNAc6S4Ac-(1→

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venusta菌株B511脂多糖中o -多糖的结构。

从俄罗斯伏尔加格勒地区Botkul湖泥中分离的中等嗜盐革兰氏阴性菌Vreelandella venusta B511中采用热水-苯酚法提取脂多糖。o -多糖（OPS）由脂多糖轻度酸水解释放，并通过化学分析、1D、2D NMR和FTIR光谱对其结构进行了表征。鉴定出OPS三糖重复单元的结构如下：→4)-β- d - manpnaca -(1→4)-β- d - manpnaca3ac -(1→3)-α- d - glcpnac6s4ac -（1→3）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".