Spectroscopic and biological investigations of a thiourea-derived turn-on sensor for Hg2+ detection and its antimicrobial activity

IF 2.8 4区化学 Q3 CHEMISTRY, INORGANIC & NUCLEAR

Transition Metal Chemistry Pub Date : 2025-11-10 DOI:10.1007/s11243-025-00689-9

Alaa Shafie, Mohammed Fareed Felemban, Faris J. Tayeb, Amal Adnan Ashour

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引用次数: 0

Abstract

In this study, a novel thiourea derivative, TH3, was synthesized and structurally characterized using FTIR and ¹H NMR spectroscopy. The fluorescence sensing behavior of TH3 was explored against a series of metal ions. Among all tested ions, TH3 exhibited a distinct and highly selective “turn-on” fluorescence response exclusively toward Hg²⁺ ions. This fluorescence enhancement is attributed to the formation of a stable TH3-Hg²⁺ complex. The Job’s plot confirmed a 1:2 binding stoichiometry between TH3 and Hg²⁺. The limit of detection (LOD) and limit of quantification (LOQ) for TH3 were calculated to be 0.0093 ppm (0.0465 µM) and 0.031 ppm, respectively., indicating a high sensitivity suitable for trace-level detection. The practical applicability of TH3 was validated by analyzing various environmental water samples (pond water, drinking water, lake water, and river water) and biological samples (human urine and blood serum), achieving excellent recovery rates ranging from 91.0 ± 0.49% to 102.2 ± 0.75%, demonstrating its reliability and robustness in complex matrices. Furthermore, the antimicrobial activity of both the free TH3 and its Hg²⁺ complex was evaluated, with both showing significant antimicrobial efficacy against tested bacterial and fungal strains.

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硫脲衍生Hg2+检测传感器的光谱和生物学研究及其抑菌活性

本研究合成了一种新型硫脲衍生物TH3，并用FTIR和1H NMR对其进行了结构表征。探讨了TH3对一系列金属离子的荧光传感行为。在所有测试的离子中，TH3表现出独特的、高度选择性的“开启”荧光反应，只针对Hg2+离子。这种荧光增强是由于形成了稳定的TH3-Hg2+络合物。约伯的情节证实了TH3和Hg2+之间的1:2结合化学计量。TH3的检出限（LOD）和定量限（LOQ）分别为0.0093 ppm（0.0465µM）和0.031 ppm。，表明适合痕量水平检测的高灵敏度。通过分析各种环境水样（池塘水、饮用水、湖泊水、河流水）和生物样品（人尿、血清），验证了TH3的实用性，回收率在91.0±0.49% ~ 102.2±0.75%之间，证明了其在复杂基质中的可靠性和鲁棒性。此外，对游离TH3及其Hg2+络合物的抑菌活性进行了评估，两者都对所测试的细菌和真菌菌株具有显著的抑菌效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transition Metal Chemistry 化学-无机化学与核化学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

1.3 months

期刊介绍： Transition Metal Chemistry is an international journal designed to deal with all aspects of the subject embodied in the title: the preparation of transition metal-based molecular compounds of all kinds (including complexes of the Group 12 elements), their structural, physical, kinetic, catalytic and biological properties, their use in chemical synthesis as well as their application in the widest context, their role in naturally occurring systems etc. Manuscripts submitted to the journal should be of broad appeal to the readership and for this reason, papers which are confined to more specialised studies such as the measurement of solution phase equilibria or thermal decomposition studies, or papers which include extensive material on f-block elements, or papers dealing with non-molecular materials, will not normally be considered for publication. Work describing new ligands or coordination geometries must provide sufficient evidence for the confident assignment of structural formulae; this will usually take the form of one or more X-ray crystal structures.