Genetic characterization of measles virus circulating in Jharkhand

IF 1.5 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2025-11-01 Epub Date: 2025-11-06 DOI:10.1016/j.ijmmb.2025.101006

Aparna Aparajita , Ashok Kumar Sharma , Nikesh Sinha , Kumari Seema , Abhay Kumar , Manju Boipai , Manoj Kumar

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引用次数: 0

Abstract

Background

Measles virus (MeV) transmission pathways can be traced via molecular surveillance based on the N-450 region of the Nucleocapsid gene of the virus. Genetic characterization of MeV can identify the circulating genotypes in a given area and provide insights into vaccine efficacy on them.

Objective

India is a vast country where molecular surveillance data on circulating MeV from all states can help gauge vaccination coverage in the elimination settings of measles. The aim of the study was genetic characterization of circulating MeV in Jharkhand and evaluate the effectiveness of vaccination drives.

Study design

Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was employed to detect MeV RNA in samples used for molecular testing. The positive RT-PCR products were sequenced, phylogenetic analysis performed, and submitted in the MeaNS2 (Measles Nucleotide Surveillance2) database.

Results

113 of the 788 molecular samples from 788 cases tested positive by RT-PCR, and sequencing was carried out on these samples. 82 samples yielded good sequences on which phylogenetic analysis was done. All sequences clustered around MANCHES.UNK94 of D8 genotype. 13 Distinct Sequence Identifier (DSId) within D8 genotype identified. The maximum cases were less than 5 years of age. No vaccine strains were identified.

Conclusions

Molecular surveillance data based on virus detection and genotyping helped to identify the circulating genotype of MeV, which is the D8 genotype in Jharkhand. Many DSIds indicate lineages of the current D8 genotype, which can be controlled with existing vaccines. Immunity gaps need to be addressed by stringent vaccination coverage.

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贾坎德邦流行麻疹病毒的遗传特征。

背景：基于病毒核衣壳基因N-450区域的分子监测可以追踪麻疹病毒（MeV）的传播途径。MeV的遗传特征可以确定特定地区的流行基因型，并为疫苗对它们的功效提供见解。目标：印度是一个幅员辽阔的国家，在这里，来自所有邦的流行MeV分子监测数据可以帮助衡量麻疹消除情况下的疫苗接种覆盖率。该研究的目的是贾坎德邦流行MeV的遗传特征，并评估疫苗接种运动的有效性。研究设计：逆转录聚合酶链式反应（RT-PCR）检测用于分子检测的样品中的MeV RNA。对阳性RT-PCR产物进行测序，进行系统发育分析，并提交至MeaNS2（麻疹核苷酸监测2）数据库。结果：788例病例的788份分子样本中，有113份经RT-PCR检测为阳性，并对这些样本进行了测序。82个样品获得了良好的序列，并对其进行了系统发育分析。所有序列都聚集在MANCHES周围。D8基因型的UNK94。在D8基因型中鉴定出13个DSId。最多的病例是5岁以下。未发现疫苗株。结论：基于病毒检测和基因分型的分子监测数据有助于确定贾坎德邦MeV的循环基因型为D8基因型。许多dsid显示当前D8基因型的谱系，这可以用现有疫苗控制。免疫缺口需要通过严格的疫苗接种覆盖来解决。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.