Comparative study of Juniperus turbinata (Cupressaceae) cone and leaf based on GC-MS-Ms analysis and biological proprieties

IF 2.2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2026-01-01 Epub Date: 2025-11-06 DOI:10.1016/j.bse.2025.105166

Mehdi Boumediene , Asmae Saih , Jihane Touhtouhe , Mohamed Akhazzane , Taoufiq Benali , Abdelhakim Bouyahya , Fatima Lakhdar , Gokhan Zengin , Khalil Hammani , Nargis Sahib

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Abstract

Essential oils (EOs) extracted from leaves (JTEO_L) and cones (JTEO_C) of Juniperus turbinata leaves were analysed to determine their chemical composition and studied in vitro their antioxidant, antidiabetic, dermatoprotective, and neuroprotective effects. The results of GC-MS-MS characterization revealed significant qualitative and quantitative differences in volatile profiles between the two parts of the plant. JTEO_C was dominated by α-pinene (81 %), followed by limonene (2.38 %), trans-verbenol (2.37 %), and α-terpinyl acetate (1.8 %), while JTEO_L contained α-pinene (20.71 %), α-terpinyl acetate (19.87 %), and β-phellandrene (15.56 %) as major components. The antioxidant assays showed varying activities, with JTEO_L showing superior radical scavenging capacity in DPPH and ABTS assays with 9.33 ± 0.55 mg TE/g and 41.16 ± 0.97 mg TE/g, respectively, while JTEO_C exhibited higher reducing power in CUPRAC and FRAP assays as 85.69 ± 1.35 mg TE/g 47.78 ± 0.27 mg TE/g, respectively. In enzyme inhibition assays, both EOs showed significant α-amylase inhibition (JTEO_C: 1.380 ± 0.019 mmol ACAE/g; JTEO_L: 1.084 ± 0.055 mmol ACAE/g) but no α-glucosidase activity. JTEO_L demonstrated superior tyrosinase inhibition (68.07 ± 3.74 mg KAE/g) compared to cone EO (17.14 ± 4.27 mg KAE/g). In cholinesterase inhibition assays, JTEO_C specifically inhibited acetylcholinesterase (7.25 ± 0.16 mg GALAE/g), while both EOs showed butyrylcholinesterase inhibition, with JTEO_L being more potent (2.85 ± 0.64 mg GALAE/g versus 1.52 ± 0.32 mg GALAE/g). Our current study suggests that J. turbinata EOs, particularly from leaves, possess a promising potential for applications in antioxidant, dermatoprotective, and neuroprotective treatments.

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基于GC-MS-Ms分析和生物学特性的刺柏球果和叶片比较研究

对刺柏叶片（JTEOL）和球果（JTEOC）中提取的精油（EOs）进行化学成分分析，并在体外研究其抗氧化、抗糖尿病、皮肤保护和神经保护作用。GC-MS-MS表征结果显示，该植物两部分的挥发性成分在定性和定量上存在显著差异。JTEOL的主要成分为α-蒎烯（81%），其次为柠檬烯（2.38%）、反式马油酚（2.37%）和α-松油酯酯（1.8%），主要成分为α-蒎烯（20.71%）、α-松油酯酯（19.87%）和β-茶树烯（15.56%）。JTEOL对DPPH和ABTS的自由基清除能力分别为9.33±0.55 mg TE/g和41.16±0.97 mg TE/g，而JTEOC对CUPRAC和FRAP的自由基清除能力分别为85.69±1.35 mg TE/g和47.78±0.27 mg TE/g。在酶抑制实验中，两种EOs均对α-淀粉酶有显著抑制作用（JTEOC: 1.380±0.019 mmol ACAE/g; JTEOL: 1.084±0.055 mmol ACAE/g），但对α-葡萄糖苷酶无明显抑制作用。JTEOL对酪氨酸酶的抑制作用（68.07±3.74 mg KAE/g）优于cone EO（17.14±4.27 mg KAE/g）。在胆碱酯酶抑制实验中，JTEOC特异性抑制乙酰胆碱酯酶（7.25±0.16 mg GALAE/g），而两种EOs均表现为丁基胆碱酯酶抑制，JTEOL更有效（2.85±0.64 mg GALAE/g vs 1.52±0.32 mg GALAE/g）。我们目前的研究表明，甲鱼叶提取物在抗氧化、皮肤保护和神经保护治疗方面具有广阔的应用前景。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.