Enhancing the clinical potential: Quality assessment of adipose tissue-derived Mesenchymal Stem/Stromal Cells for therapeutic use

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-10-24 DOI:10.1016/j.advms.2025.10.002

Monika Rytel , Magdalena Chodkowska-Michalowska , Dorota Sulejczak , Natalia Krześniak , Marzena Zychowicz , Anna Sarnowska

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引用次数: 0

Abstract

Purpose

Despite ongoing debate surrounding mesenchymal stem/stromal cells (MSCs), the number of clinical trials involving MSCs has continued to increase due to their strong therapeutic potential. These cells exhibit immunosuppressive and neuroprotective properties, making them a promising approach for treating neurodegenerative diseases. Given the growing interest in stem cell-based therapies, it is essential to establish specific protocols for manufacturing adipose-derived MSCs. This study outlines the process of obtaining an ATMP cell product from ASCs, evaluating cell quality, isolation efficiency, and optimal conditions for cryopreservation, preparation, and transport.

Materials and methods

Cells were isolated from fresh, cryopreserved, and 4°C-stored adipose tissue to evaluate how different storage conditions affect cell quality. In addition, cell viability in different transport solutions over time was examined. To evaluate the therapeutic potential of ASCs, cells were cultured in cerebrospinal fluid (CSF) and neural gene and protein expression, as well as the secretome profile, were analysed.

Results

Our findings show that isolating ASCs immediately after liposuction yields the highest viable cell count. Adipose tissue can be stored at 4 °C for up to 48 h without affecting cell population doubling time, but cryopreservation negatively impacts ASCs’ clonogenicity. For optimal long-term preservation, a medium with 5 % platelet lysate and 10 % DMSO is recommended. Additionally, we found that 0.9 % NaCl is the best transport medium for maintaining cell viability. CSF exposure induced neural markers (NG2, A2B5, Nestin, β-III-tubulin, S-100-β) and morphological changes, suggesting neural-like differentiation.

Conclusions

These findings contribute to the development of protocols for MSC therapy in neurodegenerative diseases.

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增强临床潜力：脂肪组织来源的间充质干细胞/基质细胞用于治疗的质量评估

尽管围绕间充质干细胞（MSCs）的争论仍在继续，但由于其强大的治疗潜力，涉及MSCs的临床试验数量不断增加。这些细胞表现出免疫抑制和神经保护特性，使它们成为治疗神经退行性疾病的有希望的方法。鉴于对干细胞疗法的兴趣日益增长，建立制造脂肪来源的间充质干细胞的特定方案至关重要。本研究概述了从ASCs中获得ATMP细胞产品的过程，评估了细胞质量、分离效率以及冷冻保存、制备和运输的最佳条件。材料和方法从新鲜、低温保存和4°c储存的脂肪组织中分离细胞，以评估不同储存条件对细胞质量的影响。此外，随着时间的推移，细胞活力在不同的运输溶液进行了检查。为了评估ASCs的治疗潜力，在脑脊液（CSF）中培养细胞，分析神经基因和蛋白质表达以及分泌组谱。结果吸脂后立即分离ASCs可获得最高的活细胞计数。脂肪组织可在4℃下保存48小时，不影响细胞群体倍增时间，但低温保存会对ASCs的克隆原性产生负面影响。为了获得最佳的长期保存，推荐使用含有5%血小板裂解液和10% DMSO的培养基。此外，我们发现0.9% NaCl是维持细胞活力的最佳运输培养基。脑脊液暴露诱导神经标记物（NG2、A2B5、Nestin、β- iii -微管蛋白、S-100-β）和形态学改变，提示神经样分化。结论这些发现有助于MSC治疗神经退行性疾病方案的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.