Biosynthesis of CuO-nanoparticles using extracts of Mespilus germanica, Crataegus spp., and Wild barberry and their synergistic antibacterial activity

IF 6.1 1区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

GIANT Pub Date : 2025-12-01 Epub Date: 2025-10-24 DOI:10.1016/j.giant.2025.100379

Hassan Koohestani , Ali Balooch , Hamid Staji

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Abstract

The green synthesis of nanoparticles is gaining attention as a sustainable and eco-friendly alternative to conventional chemical methods. In this study, copper oxide nanoparticles (CuONPs) were biosynthesized using leaf extracts of Mespilus germanica (M-CuO), Wild barberry (B-CuO), and Crataegus spp. (CCuO) as natural reducing and stabilizing agents. The synthesized nanoparticles were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Field-emission scanning electron microscopy (FESEM). XRD results confirmed the formation of crystalline CuO with high purity. FESEM analysis revealed that the nanoparticles were nearly spherical with size ranges of 50–70 nm (M-CuO), 60–100 nm (B-CuO), and 50–80 nm (CCuO). FTIR spectra indicated the presence of functional groups such as hydroxyl (-OH) and carbon-oxygen (CO), along with characteristic Cu-O bond vibrations in the 500–700 cm-¹ range. The antibacterial activity of the CuONPs was tested against Gram-positive and Gram-negative bacteria, showing considerable inhibition. A synergistic effect was observed when combining the different types of CuONPs, resulting in enhanced antibacterial performance. These findings highlight the potential of plant-mediated CuONPs as promising candidates for biomedical and antibacterial applications.

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德国山楂、山楂和野小檗提取物合成纳米cuo的研究及其协同抗菌活性

纳米颗粒的绿色合成作为一种可持续和环保的传统化学方法的替代品正在引起人们的关注。以德国墨斯皮乌斯（M-CuO）、野杨梅（B-CuO）和山楂（CCuO）叶提取物为天然还原剂和稳定剂，合成了氧化铜纳米颗粒（CuONPs）。采用x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和场发射扫描电镜（FESEM）对合成的纳米颗粒进行了表征。XRD结果证实形成了高纯度的CuO晶体。FESEM分析表明，纳米颗粒呈近球形，粒径范围为50 ~ 70 nm （M-CuO）、60 ~ 100 nm （B-CuO）和50 ~ 80 nm （CCuO）。FTIR光谱表明，在500-700 cm-¹范围内存在羟基（- oh）和碳氧（CO）等官能团，并伴有特征性的Cu-O键振动。CuONPs对革兰氏阳性菌和革兰氏阴性菌的抑菌活性均有明显的抑制作用。不同类型的CuONPs联合使用可产生协同效应，增强抗菌性能。这些发现突出了植物介导的CuONPs作为生物医学和抗菌应用的有希望的候选者的潜力。

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来源期刊

GIANT Multiple-

CiteScore

8.50

自引率

8.60%

发文量

审稿时长

42 days

期刊介绍： Giant is an interdisciplinary title focusing on fundamental and applied macromolecular science spanning all chemistry, physics, biology, and materials aspects of the field in the broadest sense. Key areas covered include macromolecular chemistry, supramolecular assembly, multiscale and multifunctional materials, organic-inorganic hybrid materials, biophysics, biomimetics and surface science. Core topics range from developments in synthesis, characterisation and assembly towards creating uniformly sized precision macromolecules with tailored properties, to the design and assembly of nanostructured materials in multiple dimensions, and further to the study of smart or living designer materials with tuneable multiscale properties.