Quyinboa mangrovi gen. nov., sp. nov., Quyinboa ferrireducens sp. nov., and Quyinboa sediminis sp. nov., three novel Fe(III)-reducing and nitrogen-fixing bacteria isolated from mangrove sediment, and the proposal of Quyinboaceae fam. nov. within the class Bacteroidia

IF 3.2 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-11-01 Epub Date: 2025-11-04 DOI:10.1016/j.syapm.2025.126668

Xiao-Xuan Song , Le Liu , Guan-Jun Chen , Zhenxing Xu , Zong-Jun Du

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引用次数: 0

Abstract

Three marine bacteria in Bacteroidales, designated FJH62^T, FJH65^T and FJH54^T, were isolated from mangrove sediments at the Zhangjiang River estuary in Fujian, China. The cells were Gram-stain-negative, facultatively anaerobic, non-motile and straight to curved rod-shaped. Comparative analysis of the 16S rRNA gene showed that strains FJH62^T and FJH65^T shared 88.9–89.0 % identity with Saccharicrinis carchari SS12^T, while strain FJH54^T had the maximum similarity of 88.8 % to Carboxylicivirga taeanensis MEBiC 08093^T. Whole-genome relatedness indices further distinguished these isolates from other relatives, falling below the established thresholds, with strains FJH62^T, FJH65^T, and FJH54^T identified as three distinct novel species. Phylogenetic analysis based on both 16S rRNA gene and whole genome sequences indicated that these three novel strains form a monophyletic branch within the order Bacteroidales. These isolates were distinguished from their closest relatives by the presence of MK-10 as the major respiratory quinone and phosphatidylethanolamine, aminolipids, and phospholipids as the predominant polar lipids. The major cellular fatty acids and DNA G + C content were identified as iso-C_15:0 and anteiso-C_15:0 and 38.5–39.9 %, respectively. Genomic analysis and experimental validation confirmed nitrogen-fixing and Fe(III)-reducing abilities of these three strains. Based on polyphasic characterizations, these three strains represent three novel species of a novel genus, for which the names Quyinboa mangrovi gen. nov., sp. nov. (FJH62^T = KCTC 102258^T = MCCC 1H01534^T), Quyinboa ferrireducens sp. nov. (FJH65^T = KCTC 102259^T = MCCC 1H01536^T), and Quyinboa sediminis sp. nov. (FJH54^T = KCTC 102257^T = MCCC 1H01535^T) are proposed. Furthermore, a novel family, Quyinboaceae fam. nov., is proposed within the order Bacteroidales.

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从红树林沉积物中分离到的三种新型铁还原固氮细菌——曲银波mangrovi gen. nov., sp. nov.、曲银波ferrireducens sp.和曲银波sediminis sp. nov.，以及曲银波科植物的提出。11 .在拟杆菌纲内

从福建张江口红树林沉积物中分离到3种拟杆菌门细菌，分别为FJH62T、FJH65T和FJH54T。革兰氏染色阴性，兼性厌氧，无运动，直至弯曲杆状。FJH62T和FJH65T与Saccharicrinis carchari SS12T的同源性为88.9% ~ 89.0%，FJH54T与Carboxylicivirga taeanensis MEBiC 08093T的同源性最高，为88.8%。全基因组亲缘度指数进一步将这些分离株与其他亲缘株区分开来，低于既定阈值，菌株FJH62T、FJH65T和FJH54T被鉴定为三个不同的新种。基于16S rRNA基因和全基因组序列的系统发育分析表明，这3株新菌株属于拟杆菌目单系分支。这些分离株与它们最近的亲缘菌不同，它们的主要呼吸醌是MK-10，而主要极性脂质是磷脂酰乙醇胺、氨基脂和磷脂。主要细胞脂肪酸和DNA G + C含量分别为iso-C15:0和anteiso-C15:0和38.5 - 39.9%。基因组分析和实验验证证实了这三种菌株具有固氮和还原铁（III）的能力。基于多相特征，这3个菌株代表了一个新属的3个新种，分别命名为Quyinboa mangrovi . nov., sp. nov. （FJH62T = KCTC 102258T = MCCC 1H01534T）、Quyinboa ferrireducens sp. nov. 11 （FJH65T = KCTC 102259T = MCCC 1H01536T）和Quyinboa sediminis sp. nov. 11 （FJH54T = KCTC 102257T = MCCC 1H01535T）。此外，还发现了一种新的科——曲银柏科。11 .，被提议在拟杆菌目。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: