Rapid lactic acid fermentation of vegetable waste in liquid-phase systems

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-11-01 DOI:10.1016/j.bej.2025.109989

Han Zhang , Xue Song , Jie Zhang , Yongde Liu , Tianrun Bai , Feiyue Wang , Guihua Yan , Longjie Xiao , Tianzeng Jin , Jihong Zhao

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Abstract

In this study, vegetable waste liquid (VWL) was used as a substrate to produce lactic acid (LA) through anaerobic fermentation. The effects of inoculum size, carbon-to-nitrogen ratio (C/N ratio), temperature, and initial pH on LA yield and microbial succession were systematically investigated. All parameters significantly influenced LA production, with optimized conditions (12 % inoculum, initial pH of 6, C/N ratio of 20, 35 °C) yielding maximum LA production (11.76 g/L) within 16 h, corresponding to a 53.26 % glucose conversion rate. Excessive inoculum or deviation from optimal pH inhibited LA synthesis, whereas an appropriate C/N ratio enhanced microbial activity and substrate utilization. Lactobacillus plantarum was identified as the dominant species, with its relative abundance increasing from 64.14 % to 70.33 % at 16 h before stabilizing at 71.47 % at 24 h. Its metabolic dominance strongly correlated with rapid acidification, indicating its key role in efficient LA biosynthesis. Overall, precise fermentation parameter regulation effectively alleviated LA feedback inhibition and shortened the fermentation cycle to less than 24 h, offering a sustainable approach for converting vegetable waste into valuable biochemicals.

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植物废弃物液相系统快速乳酸发酵研究

本研究以植物废液（VWL）为底物，通过厌氧发酵生产乳酸（LA）。系统研究了接种量、碳氮比（C/N）、温度和初始pH对LA产量和微生物演替的影响。所有参数均显著影响LA的产量，优化条件（接种量为12 %，初始pH为6，C/N比为20,35°C）在16 h内产生最大LA产量（11.76 g/L），对应的葡萄糖转化率为53.26% %。过量接种或偏离最佳pH会抑制LA合成，而适当的C/N比率则会增强微生物活性和底物利用率。植物乳杆菌（Lactobacillus plantarum）为优势菌种，其相对丰度在16 h时从64.14 %增加到70.33 %，在24 h时稳定在71.47 %。其代谢优势与快速酸化密切相关，表明其在高效LA生物合成中起关键作用。总体而言，精确的发酵参数调节有效地缓解了LA反馈抑制，并将发酵周期缩短至24 h以下，为将蔬菜废弃物转化为有价值的生化物质提供了一条可持续的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.