Fluorescent carbon quantum dot–based μPAD for the sensitive and selective detection of magnesium ions in human serum

IF 6.9 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2025-12-01 Epub Date: 2025-10-30 DOI:10.1016/j.snr.2025.100399

Nikhil T Madhu , Alageswari D , Anjusha P Menon , Abhishek Pathak , Aarathi Pradeep , Bipin G Nair , PV Suneesh , TG Satheesh Babu

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Abstract

Accurate determination of magnesium ions in the blood is crucial due to their association with various disease conditions, including osteoporosis, nerve disorders, cardiovascular issues, and impaired kidney function. In this work, we developed a carbon quantum dot (CQDs) based fluorometric paper analytical device (PAD) for detecting magnesium ions in human blood serum. The fluorescent CQDs were synthesized using a simple microwave-assisted method. The addition of 8-hydroxyquinoline quenches the fluorescence of the CQDs, which is regained in the presence of magnesium ions. This OFF-ON mechanism was utilized for the sensitive and selective detection of magnesium ions on the PAD. In order to deploy the PAD for testing human blood samples, a calibration plot was generated from the fluorescent intensity obtained using serum samples spiked with magnesium ions. It exhibited two linear ranges of detection with a detection limit of 3.9 µM and a limit of quantification of 13 µM. The PAD showed sensitivities of 25.93 a.u/mM and 48.52 a.u/mM for the magnesium concentration ranges from 0.76 – 1.26 mM and 1.76 – 10.76 mM, respectively. The PAD was tested and validated using volunteer blood serum samples, showing good accuracy with a relative error of <5 % from the clinical lab results. This innovative CQDs-based PAD presents a promising and practical tool for reliable and efficient magnesium ion detection in human serum, offering potential applications in healthcare and clinical diagnostics.

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荧光碳量子点μPAD灵敏选择性检测人血清中镁离子

血液中镁离子的准确测定是至关重要的，因为它们与各种疾病有关，包括骨质疏松症、神经紊乱、心血管问题和肾功能受损。在这项工作中，我们开发了一种基于碳量子点（CQDs）的荧光纸分析装置（PAD），用于检测人血清中的镁离子。采用简单的微波辅助法合成了荧光CQDs。8-羟基喹啉的加入使CQDs的荧光猝灭，而在镁离子的存在下，CQDs的荧光又恢复了。利用这种OFF-ON机制对PAD上的镁离子进行了灵敏和选择性的检测。为了部署PAD用于检测人类血液样本，使用添加了镁离子的血清样本获得的荧光强度生成了校准图。它具有两个线性检测范围，检测限为3.9µM，定量限为13µM。PAD对镁浓度范围为0.76 ~ 1.26 mM和1.76 ~ 10.76 mM的灵敏度分别为25.93和48.52 a.u/mM。使用志愿者血清样本对PAD进行测试和验证，显示出良好的准确性，与临床实验室结果的相对误差为<； 5%。这种创新的基于cqds的PAD提供了一种可靠、高效的人类血清镁离子检测工具，在医疗保健和临床诊断中具有潜在的应用前景。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.