Functional characterization of GmSRS14 in regulating root nodule development of soybean

IF 4.8 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2025-12-01 Epub Date: 2025-11-01 DOI:10.1016/j.jplph.2025.154649

Kangjun Fan , Yue Wu , Yonghua Qin , Hongzao He , Lu Lv , Gang Li , Jiao Liu , Rui Qin , Hong Liu

{"title":"Functional characterization of GmSRS14 in regulating root nodule development of soybean","authors":"Kangjun Fan , Yue Wu , Yonghua Qin , Hongzao He , Lu Lv , Gang Li , Jiao Liu , Rui Qin , Hong Liu","doi":"10.1016/j.jplph.2025.154649","DOIUrl":null,"url":null,"abstract":"<div><div>SHORT INTERNODES (SHI)-related sequence (SRS) proteins are plant-specific transcription factors that modulate hormone biosynthesis and signalling. Their contribution to legume–rhizobium symbiosis, however, remains largely unexplored. Phylogenetic and collinearity analyses of legume <em>SRS</em> genes classified 12 subclasses and revealed soybean's evolutionary relationships, including large-scale gene duplication. <em>GmSRS14</em> was specifically highly expressed in root nodules and localised in the nucleus only. Exogenous IAA modulates its expression at low concentrations (1 μM), while high concentrations (100 μM) decrease nodule expression. All ABA concentrations tested (10, 20 and 50 μM) inhibited nodule growth, nitrogenase activity and <em>GmSRS14</em> expression. Functional validation via hairy root transformation demonstrated <em>GmSRS14</em> overexpression (<em>GmSRS14</em>-OE) increased nodule number, weight, and nitrogenase activity, while <em>GmSRS14</em> silencing (<em>GmSRS14-</em>RNAi) suppressed nodulation. This study provides a new idea for breeding soybean varieties with high efficiency of nitrogen fixation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"315 ","pages":"Article 154649"},"PeriodicalIF":4.8000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161725002317","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/11/1 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

SHORT INTERNODES (SHI)-related sequence (SRS) proteins are plant-specific transcription factors that modulate hormone biosynthesis and signalling. Their contribution to legume–rhizobium symbiosis, however, remains largely unexplored. Phylogenetic and collinearity analyses of legume SRS genes classified 12 subclasses and revealed soybean's evolutionary relationships, including large-scale gene duplication. GmSRS14 was specifically highly expressed in root nodules and localised in the nucleus only. Exogenous IAA modulates its expression at low concentrations (1 μM), while high concentrations (100 μM) decrease nodule expression. All ABA concentrations tested (10, 20 and 50 μM) inhibited nodule growth, nitrogenase activity and GmSRS14 expression. Functional validation via hairy root transformation demonstrated GmSRS14 overexpression (GmSRS14-OE) increased nodule number, weight, and nitrogenase activity, while GmSRS14 silencing (GmSRS14-RNAi) suppressed nodulation. This study provides a new idea for breeding soybean varieties with high efficiency of nitrogen fixation.

查看原文本刊更多论文

GmSRS14调控大豆根瘤发育的功能研究

短节间（SHI）相关序列（SRS）蛋白是调节激素生物合成和信号传导的植物特异性转录因子。然而，它们对豆科植物-根瘤菌共生的贡献在很大程度上仍未被探索。豆类SRS基因的系统发育和共线性分析划分了12个亚类，揭示了大豆的进化关系，包括大规模的基因重复。GmSRS14在根瘤中特异高表达，且仅局限于细胞核。外源IAA低浓度（1 μM）可调节其表达，高浓度（100 μM）可降低其表达。10 μM、20 μM和50 μM的ABA浓度均能抑制根瘤生长、氮酶活性和GmSRS14表达。毛状根转化的功能验证表明，GmSRS14过表达（GmSRS14- oe）增加了根瘤数量、重量和氮酶活性，而GmSRS14沉默（GmSRS14- rnai）抑制了根瘤形成。本研究为培育高效固氮大豆品种提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.