Circular RNA ribosomal protein L15 accelerates epithelial mesenchymal transformation and tumour growth in non-small cell lung carcinoma via modulating the microRNA-518c-3p/WD repeat protein 1 axis.

IF 3.2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Archives of Physiology and Biochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-07 DOI:10.1080/13813455.2025.2578747

Gen Hu, Yun Ge, Bo Chen, Songchun Liu, Tao Wang

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引用次数: 0

Abstract

Objective: This study aimed to investigate the effects of circ-RPL15 on epithelial mesenchymal transformation (EMT) and tumour growth in non-small cell lung cancer (NSCLC).

Methods: circ-RPL15, microRNA (miR)-518c-3p and WD repeat protein 1 (WDR1) in tumour tissues were detected. Cell viability, apoptosis, migration and invasion, and EMT were analysed in A549 cells after altering circ-RPL15 or miR-518c-3p expression. The targeting relationship between miR-518c-3p with circ-RPL15 or WDR1 was verified.

Results: The results elucidated that circ-RPL15 and WDR1 levels were elevated in NSCLC tissues, while miR-518c-3p was downregulated. Suppressing circ-RPL15 or elevating miR-518c-3p suppressed cell growth and delayed EMT process. miR-518c-3p was found to target both circ-RPL15 and WDR1, and increasing WDR1 counteracted the effects of reducing circ-RPL15 on A549 cells. Downregulating circ-RPL15 suppressed tumour growth in NSCLC in vivo.

Conclusion: Shortly, circ-RPL15 accelerates EMT and tumour growth in NSCLC via modulating the miR-518c-3p/WDR1 axis.

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环状RNA核糖体蛋白L15通过调节microRNA-518c-3p/WD重复蛋白1轴加速非小细胞肺癌上皮间质转化和肿瘤生长。

目的：探讨circ-RPL15对非小细胞肺癌（NSCLC）上皮间充质转化（EMT）和肿瘤生长的影响。方法：检测肿瘤组织中circ-RPL15、microRNA (miR)-518c-3p、WD重复蛋白1 （WDR1）。在改变circ-RPL15或miR-518c-3p表达后，分析A549细胞的细胞活力、凋亡、迁移和侵袭以及EMT。验证miR-518c-3p与circ-RPL15或WDR1的靶向关系。结果：结果表明，circ-RPL15和WDR1水平在NSCLC组织中升高，miR-518c-3p水平下调。抑制circ-RPL15或升高miR-518c-3p抑制细胞生长并延迟EMT过程。发现miR-518c-3p同时靶向circ-RPL15和WDR1，并且增加WDR1抵消了减少circ-RPL15对A549细胞的影响。下调circ-RPL15在体内抑制非小细胞肺癌肿瘤生长。结论：简而言之，circ-RPL15通过调节miR-518c-3p/WDR1轴加速非小细胞肺癌的EMT和肿瘤生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.