In vitro effects of transient receptor potential channel modulators on human tuba smooth muscle

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2025-10-01 Epub Date: 2025-11-04 DOI:10.1016/j.advms.2025.11.001

Ozan Ozturk , Selin Acar-Sahan , Baris Karadas , Mustafa Sengul , Huseyin Yilmaz , Selen Bahceci , Tijen Kaya-Temiz

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引用次数: 0

Abstract

Purpose

There is relatively little data on the presence of Transient Receptor Potential (TRP) channels in human tubal tissue. Our study aimed to evaluate the contraction-relaxation responses of TRPV1 channel modulators in human tuba uterina tissue. We also aimed to investigate the presence of TRPV1 channel proteins by immunohistochemical staining with TRPV1 antibodies in tuba tissue.

Material and method

Ten patients with a mean age of 37.7 years who underwent tubal ligation for sterilization were included. Tissue samples were evaluated for in vitro contractility in a tissue bath. Changes in tuba smooth muscle isometric tension were measured in mg contraction. The basal mean tension was considered as 100 and capsaicin responses in the presence or absence of antagonist were expressed as a percentage of the basal mean tension.

Results

Concentrations of capsaicin 10⁻⁷, 10⁻⁶, 10⁻⁵, and 10⁻⁴ M; AMG 9810 10⁻⁵ M and 3x10⁻⁵ M; ruthenium red 10⁻⁵ M and 3x10⁻⁵ M were used. Relaxation response was obtained after cumulative capsaicin administration (maximum relaxation by 20 %). This relaxation was partially reversed when capsaicin was applied in the presence of AMG 9810. When capsaicin was applied in the presence of ruthenium red, a complete reversal of relaxation was observed. Immunohistochemical examination of tuba tissue using TRPV1 antibodies revealed moderate to high staining.

Conclusion

The presence of TRPV1 channel in human tuba tissue and its relaxant effect were demonstrated for the first time in this study.

查看原文本刊更多论文

瞬时受体电位通道调节剂对人大号平滑肌的体外作用。

目的：关于人类输卵管组织中瞬时受体电位（TRP）通道存在的数据相对较少。我们的研究旨在评估TRPV1通道调节剂在人子宫大管组织中的收缩-松弛反应。我们还旨在通过免疫组织化学染色，用TRPV1抗体在大号组织中研究TRPV1通道蛋白的存在。材料与方法：选取10例平均年龄37.7岁行输卵管结扎绝育术的患者。组织样品在组织浴中评估体外收缩性。在mg收缩时测量了大号平滑肌等距张力的变化。基础平均张力被认为是100，在存在或不存在拮抗剂的情况下，辣椒素反应以基础平均张力的百分比表示。结果：辣椒素浓度为10-7、10-6、10-5、10-4 M；AMG 9810 10- 5m和3x10- 5m；采用钌红10- 5m和3x10- 5m。累积辣椒素给药后获得松弛反应（最大松弛20%）。当在AMG 9810存在的情况下使用辣椒素时，这种松弛被部分逆转。当在钌红存在的情况下应用辣椒素时，观察到松弛的完全逆转。使用TRPV1抗体对大号组织进行免疫组化检查，显示中高染色。结论：本研究首次证实了人大号组织中TRPV1通道的存在及其松弛作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.