Optimizing Detection Schemes for Broadband Pump-Probe Microscopy

IF 2.1 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2026-03-18 Epub Date: 2025-11-06 DOI:10.1002/jemt.70092

Kendall Benton, Emma Orcutt, Skyler Hollinbeck, Erik M. Grumstrup

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Abstract

Although similar to more commonly implemented single wavelength approaches, broadband pump-probe or transient absorption microscopy presents unique experimental challenges due to the simultaneous requirements of a broadband probe pulse and a small sample volume. Here we provide an in-depth analysis of broadband detection schemes and their common noise sources to provide strategies for balancing the conflicting needs of high sensitivity and low probe fluence. We show that broadband pump-probe microscopy is atypically sensitive to laser shot noise and therefore, low pump on/off modulation frequencies, on the order of 100 s of Hz to a few kHz, are essential to measure small ( $~ 10^{- 3} - 10^{- 4}$ ) amplitude transient spectra while remaining in the perturbative limit.

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优化宽带泵浦探针显微镜检测方案。

虽然类似于更常见的单波长方法，宽带泵浦探针或瞬态吸收显微镜提出了独特的实验挑战，由于同时要求宽带探针脉冲和小样本体积。本文对宽带检测方案及其常见噪声源进行了深入分析，为平衡高灵敏度和低探头影响的相互冲突需求提供了策略。我们表明，宽带泵浦探针显微镜对激光射击噪声非常敏感，因此，低泵浦开/关调制频率，在100秒Hz到几kHz的数量级，对于测量小（10 - 3 - 10 - 4 $$ \sim {10}^{-3}-{10}^{-4} $$）振幅瞬态光谱是必不可少的，同时保持在摄动极限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.