[Establishing the dynamics of activity and stability of total PSA samples in semen samples on gauze, diluted with distilled water].

Q4 Medicine
A A Gusarov, V L Sidorov, O D Yagmurov, K T Momynaliev
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引用次数: 0

Abstract

The article presents the results of an experimental study of the stability of total PSA in samples from sperm dried on gauze, diluted with distilled water. The goal of the work was to establish the dynamics of activity and stability of total PSA samples in the prepared extracts. The research material included samples of seminal fluid dried on gauze. The study was carried out using a quantitative enzyme-linked immunosorbent assay. Measurements in the samples were carried out 5 times during the working day. The results obtained allowed us to prove a significant decrease in total PSA activity in extracts from sperm stains on gauze for 6 hours at room temperature (+18-20 °C). The results of the study, confirmed by statistical analysis data, can be taken into account in the case of using the quantitative enzyme immunoassay method to determine the presence of sperm on material evidence during forensic biological examinations.

[建立纱布上用蒸馏水稀释的精液样品中总PSA样品活性和稳定性的动态变化]。
本文介绍了用纱布干燥、蒸馏水稀释的精子样品中总PSA稳定性的实验研究结果。该工作的目的是建立总PSA样品在制备提取物中的活性和稳定性的动力学。研究材料包括在纱布上干燥的精液样本。本研究采用定量酶联免疫吸附法进行。在工作日内对样品进行了5次测量。获得的结果使我们能够证明,在室温(+18-20°C)下,在纱布上放置6小时的精子染色提取物中,总PSA活性显著降低。该研究结果经统计分析数据证实,可用于在法医生物学检查中使用定量酶免疫分析法确定物证上精子的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Sudebno-Meditsinskaya Ekspertisa
Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine
CiteScore
0.60
自引率
0.00%
发文量
70
期刊介绍: The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.
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