Silencing IncRNA BACE1-AS alleviates neuronal injury and neurological deficits after ischemic stroke via targeting miR-103a-3p.

IF 1.9 4区医学 Q3 CLINICAL NEUROLOGY

Neurological Research Pub Date : 2026-10-01 Epub Date: 2025-10-30 DOI:10.1080/01616412.2025.2568025

Kaijie Yu, Fang Liu, Tianrui Yu, Yanping Ding, Juyao Liu, Lianlian Lv

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引用次数: 0

Abstract

Objective: To investigate the role of lncRNA BACE1-AS in neuronal injury and neurological deficits after ischemic stroke and explore its underlying molecular mechanism.

Methods: MCAO rat model and OGD/R cell model were established. BACE1-AS expression was detected by RT-qPCR. Neurological function was evaluated by mNSS and MWM test. Inflammatory factors (TNF-α, IL-6, IL-10), neuronal injury markers (NSE, GFAP), and apoptosis-related markers (Bcl-2, Bax, Caspase-3) were detected by ELISA and RT-qPCR. Bioinformatics analysis, dual-luciferase reporter assay, and RIP assay were used to validate the targeting relationship between BACE1-AS and miR-103a-3p.

Results: BACE1-AS was significantly upregulated in both MCAO rats and OGD/R-treated SH-SY5Y cells. Silencing BACE1-AS alleviated neurological deficits, reduced pro-inflammatory cytokine levels, and inhibited neuronal apoptosis. Mechanistically, BACE1-AS targeted miR-103a-3p, and inhibiting miR-103a-3p reversed the neuroprotective effects of BACE1-AS silencing in vivo and in vitro.

Conclusion: Silencing BACE1-AS mitigates neuronal injury and neurological deficits after ischemic stroke by targeting miR-103a-3p, providing a novel therapeutic target for ischemic stroke.

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通过靶向miR-103a-3p，沉默IncRNA BACE1-AS可减轻缺血性脑卒中后的神经元损伤和神经功能缺损。

目的：探讨lncRNA BACE1-AS在缺血性脑卒中后神经元损伤和神经功能缺损中的作用，并探讨其潜在的分子机制。方法：建立MCAO大鼠模型和OGD/R细胞模型。RT-qPCR检测BACE1-AS的表达。采用mNSS和MWM试验评价神经功能。采用ELISA和RT-qPCR检测炎症因子（TNF-α、IL-6、IL-10）、神经元损伤标志物（NSE、GFAP）和凋亡相关标志物（Bcl-2、Bax、Caspase-3）。采用生物信息学分析、双荧光素酶报告基因实验和RIP实验验证BACE1-AS与miR-103a-3p的靶向关系。结果：BACE1-AS在MCAO大鼠和OGD/ r处理的SH-SY5Y细胞中均显著上调。沉默BACE1-AS可减轻神经功能缺损，降低促炎细胞因子水平，抑制神经元凋亡。在机制上，BACE1-AS靶向miR-103a-3p，抑制miR-103a-3p在体内和体外逆转了BACE1-AS沉默的神经保护作用。结论：沉默BACE1-AS可通过靶向miR-103a-3p减轻缺血性脑卒中后的神经元损伤和神经功能缺损，为缺血性脑卒中提供新的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurological Research 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

116

审稿时长

5.3 months

期刊介绍： Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.