In vitro investigation of the cytotoxic effects of various honeybee venoms on Caco-2 and T98G cells.

IF 0.9 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2025-01-01 DOI:10.22099/ijvr.2025.50439.7442

S Pehlivan, E Yarsan

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引用次数: 0

Abstract

Background: Honeybee (Apis mellifera) venom contains several biologically active peptides, including melittin, apamin, enzymes, and non-peptide components. Due to its biologically active components, honeybee venom demonstrates antibacterial, antiviral, anti-inflammatory, and cytotoxic properties.

Aims: This study aimed to investigate the cytotoxic effects of honeybee venoms collected from different provinces in Turkey on colorectal adenocarcinoma (Caco-2) and glioblastoma multiforme (T98G) cell lines.

Methods: The apamin, phospholipase A2, and melittin contents of honeybee venoms were analyzed using a high-pressure liquid chromatography (HPLC) variable wavelength detector. The viability of cells after 24-hour exposure to honeybee venoms was determined using the 3-(4,5-dimethylthiazol-2-yl) (ΜTT), neutral red (NR), and dehydrogenase leakage (LDH) assays.

Results: Content analysis showed that Mugla had the highest apamin content, while Denizli had the highest phospholipase A2 and melittin contents among the analyzed bee venoms. For Caco-2 cells, the lowest inhibitory concentration (IC₅₀) values were observed in the Denizli venom group, with MTT and LDH results of 12.42 ± 0.19 µg/ml and 8.19 ± 0.61 µg/ml, respectively. For T98G cells, these values were 5.98 ± 0.40 µg/ml and 5.04 ± 0.17 µg/ml, respectively.

Conclusion: These findings indicate that honeybee venoms from different provinces contain varying levels of apamin, phospholipase A2, and melittin. The cytotoxic effects observed on Caco-2 and T98G cell lines suggest that honeybee venom may have potential as an anticancer agent.

Abstract Image

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不同蜜蜂毒液对Caco-2和T98G细胞毒作用的体外研究。

背景：蜜蜂（Apis mellifera）的毒液含有几种生物活性肽，包括蜂毒素、维生素、酶和非肽成分。由于其生物活性成分，蜂毒具有抗菌、抗病毒、抗炎和细胞毒性。目的：本研究旨在探讨收集自土耳其不同省份的蜜蜂毒液对结直肠癌（Caco-2）和多形性胶质母细胞瘤（T98G）细胞系的细胞毒性作用。方法：采用高压液相色谱（HPLC）变波长检测器分析蜜蜂毒液中维生素a、磷脂酶A2和蜂毒素的含量。使用3-（4,5-二甲基噻唑-2-基）（ΜTT）、中性红（NR）和脱氢酶渗漏（LDH）测定暴露于蜜蜂毒液24小时后细胞的活力。结果：含量分析显示，木蜂毒液中维生素a含量最高，德尼兹利毒液中磷脂酶A2和蜂毒素含量最高。对于Caco-2细胞，蛇毒组的抑制浓度（IC50）最低，MTT和LDH分别为12.42±0.19µg/ml和8.19±0.61µg/ml。对于T98G细胞，这些值分别为5.98±0.40µg/ml和5.04±0.17µg/ml。结论：不同省份的蜜蜂毒液中含有不同水平的蜂毒素、磷脂酶A2和蜂毒素。对Caco-2和T98G细胞株的细胞毒作用提示蜂毒可能具有潜在的抗癌作用。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.