Direct detection of a target nucleic acid on a surface by spraying DNA-nano-tweezers-based biosensing molecules

IF 3.1 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2026-02-01 Epub Date: 2025-10-28 DOI:10.1016/j.ab.2025.116003

Hisakage Funabashi, Hiroya Hatano, Hirotaka Nakamura, Reiji Shigematsu, Akio Kuroda

引用次数: 0

Abstract

We propose a new concept to detect a target molecule on a solid surface directly by spraying biosensing molecules. First, a filter paper as a model of a solid surface was spotted with a target nucleic acid. Then, the biosensing molecule, a DNA-nano-tweezers-structure that exhibits a peroxidase activity recognizing the target, was sprayed with hemin as a cofactor, followed by a second spray containing luminol as a substrate for a light-glowing signal. Finally, an image was recorded using a smartphone. The images revealed that only the places where the corresponding targets were spotted glowed a blue light.

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通过喷射基于dna纳米镊子的生物传感分子直接检测表面上的目标核酸。

我们提出了一种通过喷射生物传感分子直接检测固体表面目标分子的新概念。首先，将滤纸作为固体表面的模型，并在上面标记目标核酸。然后，生物传感分子，一种dna纳米镊子结构，表现出识别目标的过氧化物酶活性，被喷上血红蛋白作为辅助因子，随后第二次喷雾含有发光氨作为发光信号的底物。最后，用智能手机拍了一张照片。图像显示，只有相应目标被发现的地方才会发出蓝光。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.