Selection of optimal transmittances with the cytophotometric two-wavelength method.

Basic and applied histochemistry Pub Date : 1988-01-01
L Galassi, B Della Vecchia
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Abstract

With the help of a computer program using Newton's iterative method, it is possible to obtain a practically instantaneous solution for the two-wavelenght method equation even for values different from 2 for the ratio k of the extinction coefficients. The influence of the extinction ratio and of the mean transmittance on the correction of the distributional error and on the propagation of stochastic and systematic errors into the computed chromophore amount has been studied. After due precautions are taken to minimize chromatic and non-specific light loss errors, the selection of the optimal wavelenght couple will basically depend only on the compromise between correction of the distributional error and the least propagation of stochastic errors into the computed chromophore amount. In many instances, the use of interference filters will be convenient, since their use will eliminate the uncertainties of monochromator readjustment and will allow evenly illuminated field to be obtained more easily, particularly in the case of large measuring fields.

双波长细胞光度法最佳透射率的选择。
在计算机程序的帮助下,利用牛顿迭代法,即使消光系数的比值k的值不等于2,也可以得到双波长方法方程的实际瞬时解。研究了消光比和平均透射率对分布误差的校正以及随机和系统误差在计算的发色团量中的传播的影响。在采取适当的预防措施以最小化色光和非特异性光损失误差之后,最佳波长对的选择基本上只取决于在校正分布误差和使随机误差在计算的发色团量中传播最小之间的折衷。在许多情况下,使用干涉滤光片将是方便的,因为它们的使用将消除单色器重新调整的不确定性,并且将允许更容易地获得均匀照明的场,特别是在大测量场的情况下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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