Integrated lipidomics and network pharmacology analysis to determine how Gu Fu Sheng Capsule improves lipid metabolism in rats with steroid-induced osteonecrosis of the femoral head

IF 3.9 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2025-11-01 Epub Date: 2024-09-26 DOI:10.1016/j.jtcme.2024.09.005

Yue Li , Ying Liu , Yingchun Li , Yang Cao , Hui Zhang , Puwei Yuan , Bo Dong , Li Shen

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引用次数: 0

Abstract

Background

Steroid-induced osteonecrosis of the femoral head (SONFH) is a prevalent refractory condition in orthopedics, often causing high disability rates. Gu Fu Sheng Capsule (GFSC) is commonly used to treat SONFH during the early and intermediate stages. However, the precise underlying mechanism of action of GFSC remains elusive.

Objective

To elucidate the mechanism through which GFSC improves lipid metabolism in rats with SONFH.

Methods

A rat model of SONFH was established by administering methylprednisolone and lipopolysaccharide. Micro-computed tomography assessed femoral head effects, while hematoxylin–eosin staining examined femoral head tissues. Pathological changes were evaluated using an automated biochemical analyzer to measure changes in serum lipid levels. Besides, quantitative lipidomics and network pharmacology were used to determine how GFSC impacts SONFH. qRT-PCR and Western blotting were performed to assess mRNA and protein expression levels of key targets.

Results

GFSC can enhance bone density and prevents surface collapse of the femoral head, improve hollow bone lacuna density, reduce adipocyte infiltration, minimize osteocyte cavities, regulate the serum lipid levels and enhance lipid metabolism in SONFH rats. Moreover, integrated lipidomics and network pharmacology suggested that GFSC affects lipid metabolism-related genes by regulating nine targets, thereby influencing four metabolites and two metabolic pathways, which may mainly be due to components in GFSC such as quercetin, tanshinone iia, berberine. Western blotting and qRT-PCR data highlighted that GFSC improves lipid metabolism by regulating TP53, HSP90AA1, and ESR1.

Conclusion

GFSC effectively retards SONFH progression, potentially attributed to its ability to regulate lipid metabolism.

Abstract Image

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综合脂质组学和网络药理学分析，探讨骨复生胶囊对激素性股骨头坏死大鼠脂质代谢的改善作用

背景：类固醇性股骨头坏死（SONFH）是骨科中一种常见的顽固性疾病，通常导致高致残率。谷复生胶囊（GFSC）通常用于治疗SONFH的早期和中期。然而，GFSC的确切作用机制仍不清楚。目的探讨GFSC改善SONFH大鼠脂质代谢的作用机制。方法采用甲基强的松龙加脂多糖建立大鼠SONFH模型。显微计算机断层扫描评估股骨头的影响，而苏木精-伊红染色检查股骨头组织。采用自动生化分析仪测量血脂水平的变化来评估病理变化。此外，定量脂质组学和网络药理学研究了GFSC对SONFH的影响。采用qRT-PCR和Western blotting检测关键靶点mRNA和蛋白的表达水平。结果gfsc能提高SONFH大鼠的骨密度，防止股骨头表面塌陷，改善空心骨陷窝密度，减少脂肪细胞浸润，减少骨细胞空洞，调节血脂水平，促进脂质代谢。此外，综合脂质组学和网络药理学表明，GFSC通过调节9个靶点影响脂质代谢相关基因，从而影响4种代谢产物和2种代谢途径，这可能主要与GFSC中槲皮素、丹参酮、小檗碱等成分有关。Western blotting和qRT-PCR数据显示，GFSC通过调节TP53、HSP90AA1和ESR1来改善脂质代谢。结论fsc有效延缓了SONFH的进展，可能与其调节脂质代谢的能力有关。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.