Enhancement mechanism of copper on heterotrophic nitrification-aerobic denitrification by cold-adapted Pseudomonas sp. NY1: Insights from genome and transcriptome

IF 8.2 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2026-02-01 Epub Date: 2025-10-28 DOI:10.1016/j.biortech.2025.133565

Yixiao Liao , Lu Yang , Tengxia He , Chunxia Zheng , Manman Zhang , Litang Lu

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Abstract

Low concentrations of copper ions (Cu²⁺) can promote the heterotrophic nitrification-aerobic denitrification (HN-AD) ability of cold-adapted Pseudomonas sp. NY1, but the exact promotion mechanisms remain unclear. In this study, nitrogen balance data revealed that the removal rates of NO₂⁻-N and total nitrogen increased by 0.37 mg/L/h and 0.28 mg/L/h after addition of 0.05 mg/L Cu²⁺. The genetic bases for cold-adapted, copper-resistant, and multiple-nitrogen metabolism pathways of strain NY1 were determined by whole genome sequencing. Transcriptome and reverse transcription-quantitative polymerase chain reaction analysis revealed that strain NY1 promoted amino acid and energy production by the upregulation of assimilatory nitrite reduction to ammonium in response to copper stress. These results provided insights into the positive effects of Cu²⁺ on HN-AD bacteria by accelerating the removal of ammonium and nitrite, and advancing the application and development of HN-AD bacteria in copper-containing and low-temperature wastewater.

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铜对冷适应假单胞菌NY1异养硝化-好氧反硝化作用的增强机制：来自基因组和转录组的见解

低浓度铜离子（Cu2+）可以促进冷适应假单胞菌NY1异养硝化-好氧反硝化（HN-AD）能力，但具体的促进机制尚不清楚。本研究氮平衡数据显示，添加0.05 mg/L Cu2+后，NO2−-N和总氮的去除率分别提高了0.37 mg/L/h和0.28 mg/L/h。通过全基因组测序，确定了菌株NY1的冷适应、抗铜和多种氮代谢途径的遗传基础。转录组和逆转录-定量聚合酶链反应分析表明，菌株NY1在铜胁迫下通过上调同化亚硝酸盐还原成铵来促进氨基酸和能量的产生。这些结果揭示了Cu2+对HN-AD菌加速去除铵盐和亚硝酸盐的积极作用，促进了HN-AD菌在含铜废水和低温废水中的应用和开发。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.