A V Fadeev, Y V Ivanov, P A Petrova, A A Perederiy, M M Pisareva, A D Moshkin, A B Komissarov, D M Danilenko, D A Lioznov
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引用次数: 0
Abstract
Introduction: Human metapneumovirus (hMPV) holds significant epidemiological importance, being a dominant cause of lower respiratory tract infections in children under two years of age and individuals over 65. Multiple infections with hMPV throughout a person's life are possible due to the antigenic and genetic variability of the virus. However, the genetic variability of hMPV circulating in Russia remains unexplored.
Aim of the study: The aim of this study was to test a protocol for whole-genome sequencing of hMPV to assess the genetic diversity of metapneumoviruses circulating in certain regions of Russia.
Materials and methods: Nasopharyngeal swabs were collected from patients of different ages with acute respiratory viral infections (ARVI) tested positive for hMPV using polymerase chain reaction (PCR). From some of the samples, viral isolates were obtained in cell culture. Whole-genome sequencing was performed on both swabs and isolates using the MiSeq Illumina platform, followed by phylogenetic analysis.
Results: For the first time in Russia, whole-genome sequencing of 44 hMPV strains circulating from 2017 to 2024 was conducted. Their genetic group affiliation was described, with the A2b2 clade shown to dominate. It was confirmed that the greatest variability among genes encoding viral surface proteins was observed in the G gene, while changes in the F gene were minimal during the studied period.
Conclusion: The study provides insights into the genetic diversity of hMPV strains circulating in various regions of the Russian Federation. Understanding the genetic variability of hMPV is crucial for comprehending viral evolution, transmission dynamics, and mechanisms of immune evasion, which influence the development of vaccines and antiviral drugs.
期刊介绍：
The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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