Functional Efficacy of Intravitreal Dental Pulp Stem Cells versus Human Umbilical Vein Endothelial Cell–Conditioned Media in Experimental Retinal Ischemia–Reperfusion Injury

IF 4.6 Q1 OPHTHALMOLOGY

Ophthalmology science Pub Date : 2025-09-18 DOI:10.1016/j.xops.2025.100941

M. Hossein Nowroozzadeh MD, Mehrnoosh Maalhagh MD, Fatemeh Sanie-Jahromi PhD, Navid Fazlinejad MD, Farid Shahrivar MD, Mohsen Farvardin MD

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Abstract

Objective

To evaluate the efficacy of intravitreal dental pulp stem cell–conditioned medium (DPSC-CM) versus human umbilical vein endothelial cell–CM (HUVEC-CM) in preserving retinal function after ischemia–reperfusion injury (IRI) in rabbits.

Design

Experimental animal study.

Subjects

Eighteen rabbits subjected to retinal IRI and randomized to receive intravitreal injections of DPSC-CM, HUVEC-CM, or balanced salt solution (BSS) (n = 6 per group).

Intervention

After induction of retinal IRI, rabbits received intravitreal injections of DPSC-CM, HUVEC-CM, or BSS. Electroretinography (ERG) was performed 7 days postinjection to assess retinal function, measuring both dark-adapted (DA) and light-adapted (LA) responses.

Main Outcome Measures

Preservation of a-wave and b-wave amplitudes on ERG as indicators of photoreceptor (PR) and bipolar cell function, respectively.

Results

Both DPSC-CM and HUVEC-CM significantly preserved b-wave amplitudes in DA ERG responses compared to BSS (P < 0.05). Dental pulp stem cell–conditioned medium demonstrated superior preservation of a-wave amplitudes, indicating enhanced PR function. Both treatments also maintained LA ERG responses. Transient mucopurulent discharge in 2 DPSC-CM–treated rabbits and a localized posterior subcapsular opacity in one BSS-treated rabbit were observed; all resolved without sequelae.

Conclusions

Intravitreal DPSC-CM and HUVEC-CM are effective in preserving retinal function after IRI, with DPSC-CM showing particular advantage in PR protection. These results support further investigation into DPSC-CM and HUVEC-CM as potential therapies for retinal ischemic conditions, such as retinal artery occlusion and anterior ischemic optic neuropathy.

Financial Disclosure(s)

The authors have no proprietary or commercial interest in any materials discussed in this article.

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玻璃体内牙髓干细胞与人脐静脉内皮细胞条件介质在实验性视网膜缺血再灌注损伤中的功能比较

目的比较玻璃体内牙髓干细胞条件培养基（DPSC-CM）与人脐静脉内皮细胞（HUVEC-CM）对兔缺血再灌注损伤（IRI）后视网膜功能的保护作用。设计实验动物研究。实验对象18只视网膜IRI兔，随机接受玻璃体内注射DPSC-CM、HUVEC-CM或平衡盐溶液（BSS）（每组n = 6）。在诱导视网膜IRI后，兔接受玻璃体内注射DPSC-CM、HUVEC-CM或BSS。注射后7天进行视网膜电图（ERG）评估视网膜功能，测量暗适应（DA）和光适应（LA）反应。ERG上a波和b波振幅的保留分别作为光感受器（PR）和双极细胞功能的指标。结果与BSS相比，DPSC-CM和HUVEC-CM均能显著保留DA ERG反应的b波振幅（P < 0.05）。牙髓干细胞条件培养基显示出优越的a波振幅保存，表明PR功能增强。两种治疗均维持了LA ERG反应。2只dpsc - cm治疗兔出现短暂性粘液脓性排出，1只bss治疗兔出现局部后囊下混浊；全部解决，无后遗症。结论玻璃体内DPSC-CM和HUVEC-CM均能有效保护IRI后的视网膜功能，其中DPSC-CM在PR保护方面表现出特别的优势。这些结果支持进一步研究DPSC-CM和HUVEC-CM作为视网膜缺血性疾病（如视网膜动脉闭塞和前缺血性视神经病变）的潜在治疗方法。作者在本文中讨论的任何材料中没有专有或商业利益。

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