Molecular characteristics and zoonotic potential of enteric protozoans in domestic small ruminants in Heilongjiang Province, Northeast China

IF 2.6 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI:10.1016/j.fawpar.2025.e00296

Meiru Hou, Xuewei Liu, Lu Zhou, Jiawang Zhou, Yuxi Zhang, Tianshuai Ma, Hongyu Qiu, Chunren Wang, Junfeng Gao

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引用次数: 0

Abstract

Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis sp. are four common zoonotic intestinal protozoa, that cause frequent foodborne and waterborne outbreaks worldwide. Despite their public health importance, epidemiological data remain scarce from Heilongjiang Province in China. Fecal samples were collected from 845 sheep and 166 goats across 13 regions of Heilongjiang Province, Northeast China. PCR-based methods were used to detect these pathogens and PCR products were sequenced to determine the species/genotypes. The overall infection rates for Cryptosporidium spp., G. duodenalis, E. bieneusi, and Blastocystis sp. were 4.15 % (42/1011), 2.67 % (27/1011), 12.15 % (127/1011), and 3.56 % (36/1011), respectively. The mixed infections with two or more protozoa occurred in 2.97 % (30/1011). The geographic location was a significant risk factor for the prevalence of Cryptosporidium spp., E. bieneusi, and Blastocystis sp. in domestic small ruminants. Four Cryptosporidium genotypes (C. xiaoi, C. ubiquitum, C. bovis, C. andersoni), seven E. bieneusi genotypes (BEB6, COS-I, CHS8, CHS7, CHG1, CHG3, J), two G. duodenalis assemblages (assemblage E, assemblage A), and six Blastocystis subtypes (ST10, ST14, ST26, ST5, ST15, ST30) were identified. This study provides critical data for developing control strategies with significant implications for zoonotic risk assessment in Heilongjiang Province.

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黑龙江省家养小反刍动物肠道原生动物的分子特征及人畜共患潜力

隐孢子虫、十二指肠贾第鞭毛虫、双氏肠胞虫和囊虫是四种常见的人畜共患肠道原虫，在世界范围内经常引起食源性和水源性暴发。尽管它们具有公共卫生重要性，但中国黑龙江省的流行病学数据仍然很少。本研究收集了黑龙江省13个地区845只绵羊和166只山羊的粪便样本。采用PCR方法对病原菌进行检测，并对PCR产物进行测序，确定其种类/基因型。隐孢子虫总感染率为4.15%(42/1011)，十二指肠棘球绦虫总感染率为2.67%(27/1011)，比氏绦虫总感染率为12.15%(127/1011)，囊胚绦虫总感染率为3.56%（36/1011）。2种及2种以上原虫混合感染占2.97%（30/1011）。地理位置是家养小反刍动物隐孢子虫、布氏易绦虫和囊虫流行的重要危险因素。共鉴定出4种隐孢子虫基因型（隐孢子虫、隐孢子虫、牛隐孢子虫、安德氏隐孢子虫），7种白氏隐孢子虫基因型（BEB6、COS-I、CHS8、CHS7、CHG1、CHG3、J）， 2种十二指肠隐孢子虫基因型（组合E、组合A）， 6种囊虫基因型（ST10、ST14、ST26、ST5、ST15、ST30）。本研究为制定控制策略提供了重要数据，对黑龙江省人畜共患病风险评估具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.