Green synthesis of antioxidant and low-toxicity gold and silver nanoparticles using floral extracts

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano Pub Date : 2025-11-01 Epub Date: 2025-09-24 DOI:10.1016/j.onano.2025.100258

Geovanna Arroyo , Yolanda Angulo , Blanca Naranjo , Fernanda Toscano , Marbel Torres Arias , Alexis Debut , Carlos Reinoso , Carina Stael , Joselyn Soria , Andrés Izquierdo

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引用次数: 0

Abstract

In this study, gold (AuNPs) and silver (AgNPs) nanoparticles were synthesized using floral extracts of H. sabdariffa and P. domesticum, aiming to develop eco-friendly nanomaterials with antioxidant and low toxicity properties. The green synthesis method produced stable nanoparticles larger than 30 nm, which were characterized by high antioxidant activity, low cytotoxicity, and minimal ecotoxic effects compared to those obtained via conventional chemical reducers (trisodium citrate and tannic acid). Among the most promising results, promising results, AuNPs and AgNPs synthesized with H. sabdariffa exhibited the highest antioxidant capacity (up to 43.13% more than P. domesticum) and negligible cytotoxicity in both A549 and HFF cell lines, with AuNPs even enhancing cell viability. AgNPs from P. domesticum showed improved resistance to UV exposure buy slightly higher cytotoxicity. In contrast, chemically synthesized NPs (Q1 and Q2) induced significant cell death and exhibited increased oxidative states under UV–Vis-NIR radiation. Toxicity tests on cyanobacteria (Fisherella musicola) confirmed the biocompatibility of green synthesis NPs, with no significant growth inhibition observed for AuNPs:Hs and AgNPs:Hs. Overall, this work confirms that anthocyanin rich floral extracts can act as effective reducing and stabilizing agents, producing multifunctional nanoparticles suitable for biomedical and cosmetic applications. These findings highlight the potential of green nanotechnology to deliver safer and more sustainable nanoparticle formulations.

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利用花卉提取物绿色合成抗氧化和低毒的金、银纳米颗粒

本研究以金花和家花提取物为原料合成了金（AuNPs）和银（AgNPs）纳米粒子，旨在开发具有抗氧化和低毒性的环保型纳米材料。绿色合成方法制备的纳米颗粒稳定，直径大于30 nm，与传统化学还原剂（柠檬酸三钠和单宁酸）相比，具有高抗氧化活性、低细胞毒性和最小生态毒性的特点。其中，最有希望的结果是，在A549和HFF细胞系中，用黄麻合成的AuNPs和AgNPs均表现出最高的抗氧化能力（比家麻高出43.13%），而细胞毒性可以忽略不计，AuNPs甚至可以提高细胞活力。家蝇AgNPs的抗紫外线能力增强，但细胞毒性略高。相比之下，化学合成的NPs （Q1和Q2）在UV-Vis-NIR辐射下诱导了显著的细胞死亡，并表现出增加的氧化状态。对蓝藻（fishella musicola）的毒性试验证实了绿色合成NPs的生物相容性，对AuNPs:Hs和AgNPs:Hs没有明显的生长抑制。总的来说，这项工作证实了花青素丰富的花卉提取物可以作为有效的还原和稳定剂，生产适合生物医学和化妆品应用的多功能纳米颗粒。这些发现突出了绿色纳米技术在提供更安全和更可持续的纳米颗粒配方方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

OpenNano Medicine-Pharmacology (medical)

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

50 days

期刊介绍： OpenNano is an internationally peer-reviewed and open access journal publishing high-quality review articles and original research papers on the burgeoning area of nanopharmaceutics and nanosized delivery systems for drugs, genes, and imaging agents. The Journal publishes basic, translational and clinical research as well as methodological papers and aims to bring together chemists, biochemists, cell biologists, material scientists, pharmaceutical scientists, pharmacologists, clinicians and all others working in this exciting and challenging area.