The pollutant naphthalene causes changes in superoxide dismutases (SODs) expression, SOD activity and lipid peroxides in the white shrimp Penaeus vannamei

IF 4.6 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-12-01 Epub Date: 2025-10-24 DOI:10.1016/j.etap.2025.104854

Laura Camacho-Jiménez, Lilia Leyva-Carrillo, Silvia Gómez-Jiménez, Gloria Yepiz-Plascencia

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引用次数: 0

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants ubiquitous in coastal areas where the shrimp Penaeus vannamei is farmed. PAHs metabolism increases the production of reactive oxygen species (ROS) and causes oxidative damage. Superoxide dismutases (SODs) are important ROS scavengers. However, SODs regulation in response to PAHs has been scarcely studied in crustaceans. In this work, we report a novel extracellular copper-zinc SOD (CuZnSOD) from P. vannamei with a predicted transmembrane region. To investigate the role of shrimp SODs in the response to PAHs, we analyzed the gene expression of CuZnSOD, cytosolic (cMnSOD) and mitochondrial manganese SOD (mMnSOD) in shrimp hepatopancreas, along with the measurement of total SOD activity and lipid peroxidation. We found that NAP induces a rapid increase in oxidative damage to lipids, while causing differential responses in the SOD gene expression and total SOD activity.

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污染物萘引起凡纳滨对虾超氧化物歧化酶（SOD）表达、SOD活性和脂质过氧化物的变化

多环芳烃（PAHs）是一种持久性有机污染物，在凡纳滨对虾养殖的沿海地区普遍存在。多环芳烃代谢增加活性氧（ROS）的产生并引起氧化损伤。超氧化物歧化酶（sod）是重要的活性氧清除剂。然而，甲壳类动物对多环芳烃对sod调控的研究却很少。在这项工作中，我们报道了一种新的细胞外铜锌超氧化物歧化酶（CuZnSOD），其预测了跨膜区域。为了研究对虾SOD在多环芳烃应答中的作用，我们分析了对虾肝胰腺CuZnSOD、细胞质SOD （cMnSOD）和线粒体锰SOD （mMnSOD）的基因表达，并测定了总SOD活性和脂质过氧化水平。我们发现NAP诱导脂质氧化损伤的快速增加，同时引起SOD基因表达和总SOD活性的差异反应。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.