Selection of the predator green lacewing Chrysoperla carnea for resistance to chlorfenapyr: stability, cross resistance, and fitness cost

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2026-01-01 Epub Date: 2025-10-22 DOI:10.1016/j.cropro.2025.107453

Muhammad Abubakar , Sarfraz Ali Shad

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引用次数: 0

Abstract

An effective way to solve the problem of resistance development in pest populations is to integrate chemical and biological control. One hurdle to this combined strategy is the susceptibility of biocontrol agents to sprayed insecticides. In this study, a population of Chrysoperla carnea (Stephens) named Pirate-Sel was selected with chlorfenapyr to determine the gain in resistance, stability, and fitness cost. Pirate-Sel strain developed significantly high resistance (306.64 fold) after 18 generations of selection when compared with the laboratory susceptible population of C. carnea. The Pirate-Sel strain did not show cross-resistance to lambda-cyhalothrin (resistance ratio, RR = 0.64), spirotetramat (RR = 0.48), and triazofos (RR = 0.94). The Pirate-Sel strain was further reared for 5 generations without selection pressure resulting in a significant decrease of its resistance from 94329.29 mg of a.i./L to 22760.64 mg of a.i./L with a decrease in resistance ratio from 306.64-fold to 73.99-folds compared to the susceptible population. Two reciprocal crosses between Pirate-Sel and Unselected strain (Unsel) of C. carnea were also maintained in the laboratory; Cross-A (Pirate-Sel ♀ × Unsel ♂) and Cross-B (Unsel ♀ × Pirate-Sel ♂). The result of the fitness experiment showed that the egg hatching (%), pupation rate (%), and fecundity of Pirate-Sel population remained statistically similar to that of the Unsel population. The mean relative fitness value of Pirate-Sel, Unsel, Susceptible, Cross-A, and Cross-B strains of C. carnea was 1.17, 1.00, 0.77, 0.89, and 0.98, respectively. In conclusion, the development of high chlorfenapyr resistance in C. carnea did not affect negatively its biology, as resistance comes with no fitness cost. Our findings provide a basic knowledge of the integration of chemical and biological control for pest management with minimal or no negative effect on natural enemies.

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捕食绿草蛉对杀虫腈抗性的选择：稳定性、交叉抗性和适应成本

化学防治与生物防治相结合是解决害虫种群抗性发展问题的有效途径。这种联合策略的一个障碍是生物防治剂对喷洒杀虫剂的易感性。本研究选择了一个名为Pirate-Sel的蝶蛹（Chrysoperla carnea (Stephens)）种群，用氯虫腈测定其抗性增益、稳定性和适合度成本。经18代筛选，与实验室易感群体相比，Pirate-Sel菌株的抗性显著提高，达到306.64倍。pira - sel菌株对高效氯氟氰菊酯（抗性比RR = 0.64）、螺虫脒（抗性比RR = 0.48）和三唑磷（抗性比RR = 0.94）无交叉抗性。在没有选择压力的情况下继续饲养5代，其抗性从94329.29 mg /L显著下降到22760.64 mg /L，抗性比从易感群体的306.64倍下降到73.99倍。在实验室中还保持了两个海盗- sel与未选择菌株（Unsel）的互交杂交；Cross-A （Pirate-Sel♀× Unsel♂）和Cross-B （Unsel♀× Pirate-Sel♂）。适应度实验结果表明，海盗船种群的卵孵化率（%）、化蛹率（%）和繁殖力与海盗船种群保持统计学上的相似。海盗- sel、Unsel、易感、交叉- a和交叉- b菌株的平均相对适合度值分别为1.17、1.00、0.77、0.89和0.98。综上所述，赤霉素对氯虫腈产生高抗性并不会对其生物学产生负面影响，因为这种抗性不需要付出适应度成本。我们的研究结果提供了化学和生物综合防治害虫管理的基本知识，对天敌的负面影响很小或没有负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.