Graphdiyne-enhanced impedimetric detection of virus-induced infections

IF 5.7 3区材料科学 Q2 CHEMISTRY, PHYSICAL

FlatChem Pub Date : 2025-11-01 Epub Date: 2025-10-15 DOI:10.1016/j.flatc.2025.100955

Rachel Rui Xia Lim , Alessandro Carvani , Adriano Ambrosi , Richard D. Webster , Alessandra Bonanni

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Abstract

Accurate detection, scaled-up testing and on-site monitoring are key attributes in effective and efficient management of pandemic outbreaks. In response to the urgent need for rapid and reliable detection of viral infections, this study investigates the use of graphdiyne—a novel two-dimensional carbon allotrope—as a platform for label-free electrochemical biosensing.

The RNA-dependent RNA polymerase (RdRp) fragment, immobilized on the graphdiyne surface, served as the probe for capturing the target gene specific to SARS-CoV-2. This biorecognition event was subsequently detected through electrochemical impedance spectroscopy.

The graphdiyne material demonstrated a strong adsorption ability with DNA molecules, which enabled a high selectivity in distinguishing the target sequence from mutant and non-complementary sequences, making the resulting genosensor applicable even when the detected virus undergoes mutations over time. A limit of detection in the nanomolar range was achieved, with a linear dynamic range of the response between 10⁻⁹ M to 10⁻⁵ M.

Coupled with the disposable printed electrodes that are portable and miniaturized sensing platforms, our developed approach can enable label-free detection to be mass-performed outside of routine laboratories.

Abstract Image

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石墨烯增强病毒诱导感染的屏障检测

准确检测、扩大检测和现场监测是有效和高效管理大流行疫情的关键因素。为了响应快速可靠检测病毒感染的迫切需求，本研究研究了石墨烯-一种新型二维碳同素异形体-作为无标签电化学生物传感平台的使用。固定在石墨烯表面的RNA依赖性RNA聚合酶（RdRp）片段作为捕获SARS-CoV-2特异性靶基因的探针。这种生物识别事件随后通过电化学阻抗谱检测。石墨烯材料对DNA分子具有很强的吸附能力，这使得在区分靶序列与突变序列和非互补序列方面具有很高的选择性，使所得到的基因传感器即使在检测到的病毒随着时间的推移发生突变时也适用。在纳摩尔范围内实现了检测极限，响应的线性动态范围在10 - 9 M到10 - 5 M之间，再加上便携式和小型化传感平台的一次性印刷电极，我们开发的方法可以使无标签检测在常规实验室之外大量进行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FlatChem Multiple-

CiteScore

8.40

自引率

6.50%

发文量

104

审稿时长

26 days

期刊介绍： FlatChem - Chemistry of Flat Materials, a new voice in the community, publishes original and significant, cutting-edge research related to the chemistry of graphene and related 2D & layered materials. The overall aim of the journal is to combine the chemistry and applications of these materials, where the submission of communications, full papers, and concepts should contain chemistry in a materials context, which can be both experimental and/or theoretical. In addition to original research articles, FlatChem also offers reviews, minireviews, highlights and perspectives on the future of this research area with the scientific leaders in fields related to Flat Materials. Topics of interest include, but are not limited to, the following: -Design, synthesis, applications and investigation of graphene, graphene related materials and other 2D & layered materials (for example Silicene, Germanene, Phosphorene, MXenes, Boron nitride, Transition metal dichalcogenides) -Characterization of these materials using all forms of spectroscopy and microscopy techniques -Chemical modification or functionalization and dispersion of these materials, as well as interactions with other materials -Exploring the surface chemistry of these materials for applications in: Sensors or detectors in electrochemical/Lab on a Chip devices, Composite materials, Membranes, Environment technology, Catalysis for energy storage and conversion (for example fuel cells, supercapacitors, batteries, hydrogen storage), Biomedical technology (drug delivery, biosensing, bioimaging)