Comparative In Vitro Evaluation of Antibacterial Activity of Ganoderma lucidum– and Psidium guajava–Loaded Drug Delivery Scaffolds Against Enterococcus faecalis Biofilms

IF 1.7 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2026-04-05 Epub Date: 2025-10-23 DOI:10.1111/aej.70026

Sheetal Ghivari, Meena Naganath, Girish Kubasad, Manohar S. Kugaji, Ulka Idate

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引用次数: 0

Abstract

This in vitro study evaluated the antibacterial efficacy of novel herbal intracanal medicaments, specifically Ganoderma lucidum (GL) and Psidium guajava (PG) extracts, incorporated into Pluronic F127 drug delivery scaffolds, against Enterococcus faecalis biofilms. Biofilms were established on standardised dentine specimens and treated with herbal-loaded hydrogels, calcium hydroxide (CH), modified triple antibiotic paste (mTAP) or saline (control). Antibacterial activity was assessed via colony-forming unit (CFU) counts, quantitative real-time PCR (qPCR), and confocal laser scanning microscopy (CLSM). Results revealed significant antibacterial effects in all medicated groups compared to control, with mTAP exhibiting the highest efficacy, followed by CH and PG. PG's performance was comparable to CH and superior to GL. The study supports the potential of PG and GL herbal-loaded scaffolds as alternative intracanal medicaments pending further in vivo research.

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灵芝和番石榴载药支架对粪肠球菌生物膜抑菌活性的体外比较研究

本体外研究评估了新型中草药肛管内药物，特别是灵芝（GL）和番石榴（PG）提取物，加入Pluronic F127给药支架，对粪肠球菌生物膜的抗菌效果。在标准化的牙本质标本上建立生物膜，并用载药水凝胶、氢氧化钙（CH）、改性三重抗生素糊剂（mTAP）或生理盐水（对照）处理。通过菌落形成单位（CFU）计数、定量实时PCR （qPCR）和共聚焦激光扫描显微镜（CLSM）评估抗菌活性。结果显示，与对照组相比，所有给药组的抗菌效果均显著，其中mTAP效果最高，其次是CH和PG， PG的性能与CH相当，优于GL。该研究支持PG和GL草药支架作为替代管内药物的潜力，有待进一步的体内研究。

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.