Effects of intratympanic administration of glucocorticoids on the guinea pig inner ear

IF 1.8 4区医学 Q2 OTORHINOLARYNGOLOGY

Auris Nasus Larynx Pub Date : 2025-12-01 Epub Date: 2025-10-22 DOI:10.1016/j.anl.2025.09.005

Akinobu Kakigi , Makoto Kinoshita , Yuki Katsutani , Toshihito Sahara , Shinji Yamashita , Kenji Kondo

{"title":"Effects of intratympanic administration of glucocorticoids on the guinea pig inner ear","authors":"Akinobu Kakigi , Makoto Kinoshita , Yuki Katsutani , Toshihito Sahara , Shinji Yamashita , Kenji Kondo","doi":"10.1016/j.anl.2025.09.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates the effects of intratympanic glucocorticoid administration on the inner ear.</div></div><div><h3>Methods</h3><div>Fifteen Hartley guinea pigs were used and divided into five groups: dexamethasone-treated (<em>n</em> = 5), contralateral dexamethasone-treated (<em>n</em> = 5), hydrocortisone-treated (<em>n</em> = 5), contralateral hydrocortisone-treated (<em>n</em> = 5), and control (<em>n</em> = 5). In the control group, 1 ml of saline was injected into the right tympanic cavity via a ventral approach. In the dexamethasone-treated group, 1 ml of dexamethasone at a concentration of 3.3 mg/ml was injected into the right tympanic cavity using the same method, while the left ear of the dexamethasone-treated group remained intact. In the hydrocortisone-treated group, 1 ml of hydrocortisone at a concentration of 50 mg/ml was injected into the right tympanic cavity using the same technique, with the left ear of the hydrocortisone-treated group also remaining intact.</div><div>To assess the effects of glucocorticoids on the inner ear, we measured a cross-sectional area analysis of the scala media at three hours post-corticosteroids treatment using optical coherence tomography (OCT). The degree of a cross-sectional area change was quantitatively measured using a video digitizer.</div></div><div><h3>Results</h3><div>Ears treated with either dexamethasone or hydrocortisone showed extension of Reissner's membrane and endolymphatic hydrops. Statistical analysis indicated that a cross-sectional area of the scala media increased significantly compared to the contralateral hydrocortisone-treated and control groups.</div></div><div><h3>Conclusions</h3><div>This is the first report to investigate the effects of intratympanic administration of glucocorticoids on the guinea pig cochlea. Dilated endolymphatic space was observed in the steroid-treated ears. Although the underlying mechanism of these histological changes remains unclear, great care must be taken when administering steroids to patients with endolymphatic hydrops-related diseases, such as Meniere's disease.</div></div>","PeriodicalId":55627,"journal":{"name":"Auris Nasus Larynx","volume":"52 6","pages":"Pages 729-734"},"PeriodicalIF":1.8000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Auris Nasus Larynx","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0385814625001415","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/10/22 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

This study investigates the effects of intratympanic glucocorticoid administration on the inner ear.

Methods

Fifteen Hartley guinea pigs were used and divided into five groups: dexamethasone-treated (n = 5), contralateral dexamethasone-treated (n = 5), hydrocortisone-treated (n = 5), contralateral hydrocortisone-treated (n = 5), and control (n = 5). In the control group, 1 ml of saline was injected into the right tympanic cavity via a ventral approach. In the dexamethasone-treated group, 1 ml of dexamethasone at a concentration of 3.3 mg/ml was injected into the right tympanic cavity using the same method, while the left ear of the dexamethasone-treated group remained intact. In the hydrocortisone-treated group, 1 ml of hydrocortisone at a concentration of 50 mg/ml was injected into the right tympanic cavity using the same technique, with the left ear of the hydrocortisone-treated group also remaining intact.

To assess the effects of glucocorticoids on the inner ear, we measured a cross-sectional area analysis of the scala media at three hours post-corticosteroids treatment using optical coherence tomography (OCT). The degree of a cross-sectional area change was quantitatively measured using a video digitizer.

Results

Ears treated with either dexamethasone or hydrocortisone showed extension of Reissner's membrane and endolymphatic hydrops. Statistical analysis indicated that a cross-sectional area of the scala media increased significantly compared to the contralateral hydrocortisone-treated and control groups.

Conclusions

This is the first report to investigate the effects of intratympanic administration of glucocorticoids on the guinea pig cochlea. Dilated endolymphatic space was observed in the steroid-treated ears. Although the underlying mechanism of these histological changes remains unclear, great care must be taken when administering steroids to patients with endolymphatic hydrops-related diseases, such as Meniere's disease.

查看原文本刊更多论文

鼓腔内给药糖皮质激素对豚鼠内耳的影响。

目的：探讨鼓室内给药糖皮质激素对内耳的影响。方法：选取15只Hartley豚鼠，随机分为5组：地塞米松组（n = 5）、对侧地塞米松组（n = 5）、氢化可的松组（n = 5）、对侧氢化可的松组（n = 5）和对照组（n = 5）。对照组经腹侧入路将生理盐水1 ml注入右鼓室。地塞米松治疗组，同样方法将浓度为3.3 mg/ml的地塞米松1 ml注射到右鼓室，而地塞米松治疗组的左耳保持完整。在氢化可的松处理组，使用相同的技术将浓度为50 mg/ml的氢化可的松1 ml注射到右鼓室，氢化可的松处理组的左耳也保持完整。为了评估糖皮质激素对内耳的影响，我们使用光学相干断层扫描（OCT）测量了糖皮质激素治疗后3小时scala介质的横截面积分析。横截面积变化的程度用视频数字化仪定量测量。结果：用地塞米松或氢化可的松治疗的耳部均出现雷氏膜扩张和内淋巴积液。统计分析表明，与对侧氢化可的松治疗组和对照组相比，中膜横截面积显著增加。结论：这是首次报道鼓室内给药糖皮质激素对豚鼠耳蜗的影响。类固醇治疗后耳内淋巴间隙扩大。虽然这些组织学改变的潜在机制尚不清楚，但在给患有内淋巴水肿相关疾病（如梅尼埃病）的患者使用类固醇时必须非常小心。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Auris Nasus Larynx 医学-耳鼻喉科学

CiteScore

3.40

自引率

5.90%

发文量

169

审稿时长

30 days

期刊介绍： The international journal Auris Nasus Larynx provides the opportunity for rapid, carefully reviewed publications concerning the fundamental and clinical aspects of otorhinolaryngology and related fields. This includes otology, neurotology, bronchoesophagology, laryngology, rhinology, allergology, head and neck medicine and oncologic surgery, maxillofacial and plastic surgery, audiology, speech science. Original papers, short communications and original case reports can be submitted. Reviews on recent developments are invited regularly and Letters to the Editor commenting on papers or any aspect of Auris Nasus Larynx are welcomed. Founded in 1973 and previously published by the Society for Promotion of International Otorhinolaryngology, the journal is now the official English-language journal of the Oto-Rhino-Laryngological Society of Japan, Inc. The aim of its new international Editorial Board is to make Auris Nasus Larynx an international forum for high quality research and clinical sciences.