Automated spatial analysis reveals proximity of human leukocyte antigen class I-positive tumor cells and CD8-positive cells of the tumor microenvironment in stage I seminoma

IF 3.7 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI:10.1016/j.prp.2025.156270

Takuto Ogasawara , Terufumi Kubo , Kenta Sasaki , Naoki Shijubou , Rena Morita , Kenji Murata , Kohei Hashimoto , Tomohide Tsukahara , Hiroko Asanuma , Toshihiko Torigoe , Reiko Watanabe , Naoya Masumori , Yoshihiko Hirohashi

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引用次数: 0

Abstract

Background

The testis is an immune-privileged organ due to its lack of functional human leukocyte antigen (HLA) class I expression. However, the expression status of HLA class I in testicular seminoma, which is characterized histologically by lymphocytic infiltration, remains unclear.

Materials and methods

We performed immunohistochemistry to evaluate HLA class I expression and its spatial relationship with CD8-positive cells in 20 cases of stage I seminoma. Each specimen was scored manually and further analyzed using the HALO image analysis platform.

Results

Manual scoring and HALO analysis showed 80 % concordance. HLA class I expression was positive in 13 cases (65 %). Tumors were classified into three categories based on the expression levels. The number of infiltrating CD8-positive cells was significantly correlated with HLA class I expression. Spatial analysis revealed that tumors with higher HLA class I expression had shorter distances between HLA class I–positive tumor cells and CD8-positive cells.

Conclusion

Seminoma cells expressing HLA class I tended to be located in close proximity to CD8-positive T cells, suggesting a spatial relationship that might influence the tumor immune microenvironment.

查看原文本刊更多论文

自动空间分析揭示了I期精原细胞瘤中人类白细胞抗原I类阳性肿瘤细胞和cd8阳性肿瘤微环境细胞的接近性。

背景：由于缺乏功能性人类白细胞抗原（HLA） I类表达，睾丸是一种免疫特权器官。然而，HLA I类在以淋巴细胞浸润为组织学特征的睾丸精原细胞瘤中的表达状况尚不清楚。材料和方法：应用免疫组织化学方法评价20例I期精原细胞瘤患者HLA I类表达及其与cd8阳性细胞的空间关系。每个标本手工评分，并使用HALO图像分析平台进一步分析。结果：手工评分与HALO分析的符合率为 %。HLA I类阳性13例（65 %）。根据表达水平将肿瘤分为三类。cd8阳性细胞浸润数与HLA I类表达显著相关。空间分析显示，HLA I类表达较高的肿瘤，HLA I类阳性肿瘤细胞与cd8阳性细胞之间的距离较短。结论：表达HLA I类的精原细胞瘤细胞倾向于靠近cd8阳性T细胞，可能存在影响肿瘤免疫微环境的空间关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.