Development and application of functional markers based on the CmAPRR2 gene for peel color improvement in Muskmelon (Cucumis melo).

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-10-20 eCollection Date: 2025-11-01 DOI:10.1007/s11032-025-01604-x

Rong Fan, Meihua Li, Yongbing Zhang, Bin Liu, Xuejun Zhang, Chaohong Deng, Changchun Fang, Wei Liu, Yong Yang

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引用次数: 0

Abstract

Peel is a crucial determinant of consumer preference and market value in melons, significantly influencing commercial viability. To overcome limitations in conventional breeding for melon peel color improvement, we used 'VZX' (green peel) and 'HP' (yellow peel) as parental materials to construct an F₂ (n = 414) and RIL (n = 116) populations for genetic dissection and mapping. Genetic analysis revealed that green peel color is a dominant trait controlled by a single gene. Using phenotypic data and whole-genome resequencing of the RIL population, we constructed a high-density genetic landscape and identified a major-effect quantitative trait locus (QTL) spanning 77 kb (499.6-576.5 kb on chromosome 4), with a LOD score of 14.85, explaining 41.76% of peel color phenotypic variation. Bulked segregant analysis (BSA) confirmed mapping accuracy. Within this interval, 13 candidate genes were annotated via Gene annotation analysis, including EVM0008709, which encodes the APRR2 transcription factor previously associated with chlorophyll metabolism and pigment regulation in melon, watermelon, and other crops. Three foreground selection markers (RC1, RC2, and RC3) were developed based on EVM0008709 and demonstrated 100% validation accuracy in both the F₂ and RIL populations. Additionally, leveraging resequencing data from 'VZX' and 'HP', we designed 204 kompetitive allele-specific PCR (KASP) markers, with 141 exhibiting robust genotyping performance for background selection. Using marker-assisted backcrossing (MABC) with 'HP' as the donor and 'VZX' as the recipient, we developed a novel yellow peel germplasm, 'YVZX', which retains the superior flesh quality of 'VZX'. This study establishes an integrated germplasm innovation pipeline spanning from gene mapping to marker development, and molecular breeding, thereby providing a reproducible technical framework for enhancement of melon quality traits.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01604-x.

查看原文本刊更多论文

基于CmAPRR2基因的甜瓜果皮颜色改良功能标记的开发与应用。

果皮是消费者偏好和甜瓜市场价值的关键决定因素，对商业可行性有重大影响。为了克服传统育种在甜瓜果皮颜色改良方面的局限性，我们以“VZX”（绿果皮）和“HP”（黄果皮）为亲本材料，构建了F2 （n = 414）和RIL （n = 116）群体进行遗传解剖和定位。遗传分析表明，果皮颜色为单基因控制的显性性状。利用表型数据和RIL群体的全基因组重测序，构建了高密度遗传景观，并确定了一个全长77 kb（4号染色体499.6 ~ 576.5 kb）的主要效应数量性状位点（QTL）， LOD评分为14.85，解释了41.76%的果皮颜色表型变异。本体分离分析（BSA）证实了制图的准确性。在这段时间内，通过基因注释分析，对13个候选基因进行了注释，其中包括EVM0008709，该基因编码APRR2转录因子，此前在甜瓜、西瓜和其他作物中与叶绿素代谢和色素调控相关。基于EVM0008709开发了3个前景选择标记（RC1、RC2和RC3），在F2和RIL群体中均证明了100%的验证准确性。此外，利用“VZX”和“HP”的重测序数据，我们设计了204个竞争性等位基因特异性PCR （KASP）标记，其中141个在背景选择中表现出强大的基因分型性能。以‘HP’为供体，‘VZX’为受体，采用标记辅助回交（MABC）技术，获得了一种保留了‘VZX’优良果肉品质的黄皮种质YVZX。本研究建立了从基因定位到标记开发、分子育种的综合种质创新管道，为提高甜瓜品质性状提供了可复制的技术框架。补充信息：在线版本包含补充资料，提供地址为10.1007/s11032-025-01604-x。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.