Comparative Study of [¹⁸F]DPA714 and [¹⁸F]FDG PET Tracers in an Experimental Model of Pulmonary Tuberculosis.

IF 2.5 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Molecular Imaging and Biology Pub Date : 2025-12-01 Epub Date: 2025-10-21 DOI:10.1007/s11307-025-02057-6

M A Stammes, M P M Vierboom, C C Sombroek, J Bakker, L Meijer, R A W Vervenne, S O Hofman, E Nutma, I Kondova, A D Windhorst, J A M Langermans, F A W Verreck

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引用次数: 0

Abstract

Purpose: Tuberculosis (TB) continues to afflict global health. Therefore, a deeper understanding of the host response mechanisms that underly pathogenesis versus disease control upon infection with Mycobacterium tuberculosis (Mtb) is required to leverage the development of improved therapeutic or prophylactic TB treatment regimens. In the present work positron emission tomography (PET) using [¹⁸F]DPA714 is piloted as a tracer of the mitochondrial translocator protein TSPO that mainly targets macrophages.

Procedures: We compared two tracers: [¹⁸F]DPA714 to the widely applied marker [¹⁸F]FDG to visualize the development of experimental pulmonary TB in three rhesus monkeys (Macaca mulatta), that were infected with Mtb by repeated low dose exposure. Next to baseline recordings prior to infectious challenge, two PETs at a two-weeks interval were acquired early after the manifestation of TB infection for each of the respective tracers.

Results & conclusions: Here, we demonstrate that both PET tracers detected Mtb infection. The inflammatory response tracked by [¹⁸F]FDG progressively increased in line with the developing TB pathology, while [¹⁸F]DPA714 showed a transient signal in lungs and lung-draining hilar lymph nodes. This study underpins the potential value of different tracers to investigate cellular and molecular host response cascades in experimental medicine settings, in this case, into a (transient) local involvement of myeloid immune cell activation versus inflammation-associated glucose consumption in pulmonary TB.

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[18F]DPA714与[18F]FDG PET示踪剂在肺结核实验模型中的比较研究。

目的：结核病继续困扰着全球健康。因此，需要更深入地了解感染结核分枝杆菌（Mtb）时宿主反应机制的潜在发病机制和疾病控制机制，以促进改进的治疗或预防性结核病治疗方案的发展。在本研究中，使用[18F]DPA714的正电子发射断层扫描（PET）被引导作为主要靶向巨噬细胞的线粒体转运蛋白TSPO的示踪剂。方法：我们比较了两种示踪剂[18F]DPA714和广泛应用的标记物[18F]FDG，以观察通过反复低剂量暴露感染Mtb的3只恒河猴（Macaca mulatta）实验性肺结核的发展。除了感染前的基线记录外，在结核感染出现后的早期，每隔两周对每种示踪剂进行两次pet检测。结果与结论：在这里，我们证明两种PET示踪剂都能检测到结核分枝杆菌感染。[18F]FDG追踪的炎症反应随着TB病理的发展而逐渐增加，而[18F]DPA714在肺和肺门淋巴结中显示一过性信号。这项研究支持了不同示踪剂在实验医学环境中研究细胞和分子宿主反应级联反应的潜在价值，在这种情况下，在肺结核中（短暂）局部参与骨髓免疫细胞激活与炎症相关的葡萄糖消耗。

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来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.