C13MP, a method using C13 isotope labeling to measure plant photosynthetic rate precisely.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-10-20 eCollection Date: 2025-11-01 DOI:10.1007/s11032-025-01601-0

Qing Xiong, Ruijie Mao, Yizhe Lu, Yanwen Wang, Zhengchang Wang, Zuxin Zhang, Zhihong Li, Jie Liu, Lei Liu

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引用次数: 0

Abstract

Photosynthesis is the cornerstone of life on earth. Precisely evaluating the photosynthetic rate is critical to uncovering its underlying mechanisms. Numerous methods and tools have been developed to assess plant photosynthetic rate. However, these methods typically measure the carbon assimilation rate indirectly during photosynthesis, highlighting the need for a new method to determine plant photosynthetic rate precisely. This study developed an easy and reliable method using ¹³C isotope-labeled CO₂ and isotope element spectrometer technology to measure plant photosynthetic rate (C13MP). C13MP can precisely quantify the abundance of assimilated carbon by photosynthesis in plants. C13MP can also evaluate the carbon flow from leaves to the other organs by tracking the ¹³C isotope. The photosynthetic rates determined with C13MP were significantly correlated with maize biomass-related and yield-related phenotypes, suggesting the reliability of this method. In conclusion, C13MP can easily, precisely, and reliably evaluate carbon assimilation in various parts of the plant, providing a new method for measuring plant photosynthetic rate.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01601-0.

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C13MP，一种利用C13同位素标记精确测量植物光合速率的方法。

光合作用是地球上生命的基石。精确评估光合速率对于揭示其潜在机制至关重要。许多方法和工具已经开发出来评估植物光合速率。然而，这些方法通常是间接测量光合作用过程中的碳同化速率，因此需要一种新的方法来精确测定植物光合速率。本研究利用13C同位素标记CO2和同位素元素光谱仪技术，建立了一种简便、可靠的植物光合速率（C13MP）测定方法。C13MP可以精确地量化植物光合作用吸收碳的丰度。C13MP还可以通过跟踪13C同位素来评估从叶片到其他器官的碳流。C13MP测定的光合速率与玉米生物量相关表型和产量相关表型显著相关，表明该方法的可靠性。综上所述，C13MP可以方便、准确、可靠地评估植物各部位的碳同化，为测量植物光合速率提供了一种新的方法。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01601-0。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.