An Experimental Study on the Widely Used Chemotherapeutic Docetaxel: Does It Induce Inflammation, Ischemia, and Neurodegeneration in the Eye, Causing Dry Eye and Blurred Vision in a Real-Life Scenario?

IF 2 4区医学 Q3 OPHTHALMOLOGY

Current Eye Research Pub Date : 2025-10-22 DOI:10.1080/02713683.2025.2575785

Ayse Ipek Akyuz Unsal, Fadime Kahyaoglu, Sayime Aydin Eroglu, Tuna Onal, Bilgin Demir, Sabri Barutca, Buket Demirci

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Abstract

Purpose: Chemotherapy protocols for lung, breast, and prostate cancer include Docetaxel (DTX). Several case series have reported ophthalmic side effects of DTX, such as epiphora and blurred vision, which significantly affect quality of life. This experimental study aims to investigate the potential histopathological impacts of DTX on ocular structures.

Methods: A DTX-treated group consisting of male Wistar rats aged 6 to 8 months (n = 7) received intraperitoneal administration of 10 mg/kg DTX three times on days 0, 8, and 15. A control group (n = 6) received weekly intraperitoneal injections of physiological saline. On the 22nd day, ocular tissues were evaluated using hematoxylin and eosin (H&E) staining, along with immunohistochemical analysis of iNOS, eNOS, IL-6, TGF-β, VEGF, and TUNEL markers.

Results: The main outcomes observed through H&E evaluation revealed corneal neovascularization, inflammatory cells with cystic dilatations in the lacrimal gland, and degeneration of the retinal nerve fiber layer. DTX treatment significantly increased the levels of iNOS, eNOS, IL-6, TGF-β, VEGF, and apoptosis markers compared to the control group.

Conclusions: This experimental study demonstrated that DTX induces inflammation and ischemia in ocular tissues, as shown in histopathological sections. Given the rising incidence of cancer and the related use of chemotherapeutics, it is crucial for ophthalmologists to recognize the ocular side effects of drugs like DTX in order to enhance the quality of life for cancer patients undergoing chemotherapy.

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广泛使用的化疗药物多西他赛的实验研究：在现实生活中，多西他赛是否会诱发眼部炎症、缺血和神经退行性变，导致干眼和视力模糊？

目的：肺癌、乳腺癌和前列腺癌的化疗方案包括多西他赛（DTX）。一些病例系列报道了DTX的眼部副作用，如眼珠脱落和视力模糊，严重影响生活质量。本实验旨在探讨DTX对眼部结构的潜在组织病理学影响。方法：DTX治疗组6 ~ 8月龄雄性Wistar大鼠（n = 7），分别于第0、8、15天腹腔注射DTX 10 mg/kg 3次。对照组（n = 6）每周腹腔注射生理盐水。第22天，采用苏木精和伊红（H&E）染色评估眼组织，同时免疫组织化学分析iNOS、eNOS、IL-6、TGF-β、VEGF和TUNEL标志物。结果：H&E评价的主要结果为角膜新生血管、泪腺炎性细胞囊性扩张、视网膜神经纤维层变性。与对照组相比，DTX治疗显著提高了iNOS、eNOS、IL-6、TGF-β、VEGF和凋亡标志物的水平。结论：本实验研究表明，DTX可引起眼部组织炎症和缺血，病理切片显示。随着癌症发病率的上升和化疗药物的使用，眼科医生认识到DTX等药物对眼部的副作用，以提高化疗癌症患者的生活质量至关重要。

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来源期刊

Current Eye Research 医学-眼科学

CiteScore

4.60

自引率

0.00%

发文量

163

审稿时长

12 months

期刊介绍： The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.