{"title":"Clinical pathological significance and biological functions of APIP in hepatocellular carcinoma.","authors":"Cong Yu, Jiang-Hua Huang, Rui Zhang, Kang-Lai Wei, Zhen-Bo Feng","doi":"10.1186/s12935-025-04018-z","DOIUrl":null,"url":null,"abstract":"<p><strong>Background & aims: </strong>Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally, characterized by high morbidity and mortality. This study aims to investigate the clinical and pathological significance of Apaf-1-interacting protein (APIP) expression in HCC, assess its biological effects on HCC cells, and explore the underlying mechanisms.</p><p><strong>Methods: </strong>Internal tissue samples were analyzed to determine APIP expression and its clinical relevance in HCC using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC). External datasets were employed for verification. Experiments investigating cell proliferation, cell cycle progression, apoptosis, migration, and invasion were performed to understand the biological role and molecular mechanisms of APIP in HCC cells. Additionally, upstream transcription factors regulating APIP expression were explored.</p><p><strong>Results: </strong>APIP expressionwas significantly higher in HCC tissues compared to adjacent non-tumor tissues, effectively distinguishing HCC from normal liver tissues. APIP expression correlated with age, gender, and poor prognosis in patients with HCC. Furthermore, downregulation of APIP promoted apoptosis and inhibited proliferation, migration, and invasion of HCC cells. A regulatory relationship between APIP and the transcription factor Serum Response Factor (SRF) was also identified.</p><p><strong>Conclusion: </strong>APIP significantly contributes to the initiation and progression of HCC by mediating cell proliferation, apoptosis, migration, and invasion. Therefore, APIP may serve as a potential diagnostic and prognostic biomarker for HCC.</p>","PeriodicalId":9385,"journal":{"name":"Cancer Cell International","volume":"25 1","pages":"368"},"PeriodicalIF":6.0000,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Cell International","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12935-025-04018-z","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background & aims: Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally, characterized by high morbidity and mortality. This study aims to investigate the clinical and pathological significance of Apaf-1-interacting protein (APIP) expression in HCC, assess its biological effects on HCC cells, and explore the underlying mechanisms.
Methods: Internal tissue samples were analyzed to determine APIP expression and its clinical relevance in HCC using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC). External datasets were employed for verification. Experiments investigating cell proliferation, cell cycle progression, apoptosis, migration, and invasion were performed to understand the biological role and molecular mechanisms of APIP in HCC cells. Additionally, upstream transcription factors regulating APIP expression were explored.
Results: APIP expressionwas significantly higher in HCC tissues compared to adjacent non-tumor tissues, effectively distinguishing HCC from normal liver tissues. APIP expression correlated with age, gender, and poor prognosis in patients with HCC. Furthermore, downregulation of APIP promoted apoptosis and inhibited proliferation, migration, and invasion of HCC cells. A regulatory relationship between APIP and the transcription factor Serum Response Factor (SRF) was also identified.
Conclusion: APIP significantly contributes to the initiation and progression of HCC by mediating cell proliferation, apoptosis, migration, and invasion. Therefore, APIP may serve as a potential diagnostic and prognostic biomarker for HCC.
期刊介绍：
Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques.
The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors.
Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.
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