Construction of aptamer functionalized camptothecin incorporated chitosan-palladium/gold nanoparticles delivery improved photothermal activity and anti-lung carcinoma cells

IF 4.5 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2025-10-22 DOI:10.1007/s10856-025-06936-w

Zheng Wang, Minhua Ye, Chunguo Wang, Dehua Ma, Jian Zhang, Fang Fang

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Abstract

This study aimed to develop a versatile aptamer-conjugated, photothermal responsive camptothecin (CPT)-loaded chitosan-bimetallic (Pd/Au) nanoparticles (Ap-CH-CPT-Pd/Au NPs) to enhance cytotoxicity in lung cancerous NCI-H446 and H1299 cells. The CH-CPT-Pd/Au NPs exhibited polydispersity with a diameter of 33.87 ± 2.23 nm. FTIR investigation revealed the presence of chitosan and camptothecin in chitosan-camptothecin-palladium/gold nanoparticles. The 2θ of CH-CPT-Pd/Au corresponded to chitosan and palladium/gold. The Ap-CH-CPT-Pd/Au NPs (180 μg/mL) subjected to near-infrared (NIR) treatment elevated the temperature to over 50 °C. The optimum CPT concentration was 0.075% in CH-CPT-Pd/Au, demonstrating a hydrodynamic diameter of 113.12 ± 16.78 nm, a drug loading efficiency (DLE) of 10.89 ± 0.53%, and a drug encapsulation efficiency (DEE) of 63.97 ± 4.21%. A CPT release rate of 7.23 ± 3.25% was recorded at pH = 5.4 after 74 h. In addition, NIR+Ap-CH-CPT-Pd/Au NPs exhibited negligible toxicity to red blood cells (RBCs). However, enhanced cytotoxicity in NCI-H446 and H1299 lung carcinoma cells is achieved through the induction of oxidative stress-mediated apoptosis.

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适体功能化喜树碱结合壳聚糖-钯/金纳米颗粒的构建提高了光热活性和抗肺癌细胞。

本研究旨在开发一种多功能的适体偶联、光热响应的喜树碱（CPT）负载壳聚糖双金属（Pd/Au）纳米颗粒（Ap-CH-CPT-Pd/Au NPs），以增强肺癌NCI-H446和H1299细胞的细胞毒性。CH-CPT-Pd/Au NPs具有多分散性，直径为33.87±2.23 nm。红外光谱研究表明壳聚糖-喜树碱-钯金纳米颗粒中含有壳聚糖和喜树碱。CH-CPT-Pd/Au的2θ对应壳聚糖和钯/金。近红外（NIR）处理的Ap-CH-CPT-Pd/Au NPs （180 μg/mL）温度升高至50℃以上。在CH-CPT-Pd/Au中，CPT的最佳浓度为0.075%，其水动力直径为113.12±16.78 nm，载药效率（DLE）为10.89±0.53%，包封效率（DEE）为63.97±4.21%。在pH = 5.4条件下，经74 h， CPT释放率为7.23±3.25%。此外，NIR+Ap-CH-CPT-Pd/Au NPs对红细胞（rbc）的毒性可忽略不计。然而，NCI-H446和H1299肺癌细胞的细胞毒性增强是通过诱导氧化应激介导的凋亡来实现的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.