Dual-signal amplified SERS sensing based on aptamer-modified Au/Fe3O4 nanohybrids and Ag NPs-functionalized bacterial cellulose film for on-site marine toxin analysis

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-10-13 DOI:10.1016/j.snb.2025.138929

Jian Chen , Jiaying Yang , Yanbin Lu , Zhiyuan Dai , Huan Li

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引用次数: 0

Abstract

Okadaic acid (OA), a diarrhetic shellfish toxin, poses severe health risks to humans and causes substantial economic losses in aquaculture, creating an urgent need for rapid, sensitive, and field-deployable detection technologies for tracing its contamination in shellfish. Herein, we proposed a surface-enhanced Raman scattering (SERS) sensing method for OA in shellfish. The system was constructed by integrating Raman-active aptamer-modified gold/magnetic nanohybrids (Apt-Au/Fe₃O₄ NHs) with a complementary sequence-modified silver nanoparticle-functionalized bacterial cellulose film (CS-Ag NPs@BC-film). Upon the introduction of OA, it competitively bound with the Apt, leaving the CS-Ag NPs@BC-film after magnetic separation, thereby inducing a reduction in the Raman signal. Sensitive quantification of OA was achieved by measuring the difference in the Raman signal, achieving a detection limit as low as 2.82 × 10⁻² nM (S/N = 3). Analysis of the spiked real samples demonstrated good accuracy and precision of the method. Compared with commercial ELISA kits, the method presented with comparable detection results while offering simplified operational workflows. These findings highlight its potential as a practical tool for monitoring hazardous contaminants in aquatic environments and ensuring seafood safety.

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基于适配体修饰Au/Fe3O4纳米杂化体和Ag nps功能化细菌纤维素膜的双信号放大SERS传感用于海洋毒素现场分析

冈田酸（OA）是一种腹泻性贝类毒素，对人类健康构成严重威胁，并在水产养殖中造成重大经济损失，迫切需要快速、敏感和可现场部署的检测技术，以追踪其在贝类中的污染。在此，我们提出了一种表面增强拉曼散射（SERS）检测贝类OA的方法。该系统是通过将拉曼活性核酸适体修饰的金/磁性纳米杂化物（Apt-Au/Fe3O4 NHs）与互补序列修饰的银纳米颗粒功能化细菌纤维素膜（CS-Ag NPs@BC-film）整合而成的。在引入OA后，它与Apt竞争性结合，在磁分离后离开CS-Ag NPs@BC-film，从而引起拉曼信号的降低。通过测量拉曼信号的差异，实现了OA的灵敏定量，检测限低至2.82 × 10−2 nM （S/N = 3）。对加标样品的分析表明，该方法具有良好的准确度和精密度。与市售ELISA试剂盒相比，该方法具有可比较的检测结果，同时简化了操作流程。这些发现突出了它作为监测水生环境中有害污染物和确保海产品安全的实用工具的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.